Analytical Data
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基因名
PPM1B
- Application
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别名
PPM1B；PP2CB；Protein phosphatase 1B
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O75688
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表达区间
1-192aa
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氨基酸序列
MSIVLVCFSNAPKVSDEAVKKDSELDKHLESRVEEIMEKSGEEGMPDLAHVMRILSAENIPNLPPGGGLAGKRNVIEAVYSRLNPHRESDGASDEAEESGSQGKLVEALRQMRINHRGNYRQLLEEMLTSYRLAKVEGEESPAEPAATATSSNSDAGNPVTMQESHTESESGLAELDSSNEDAGTKMSGEKI
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分子量
36.6 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PPM1B, a member of the protein phosphatase 2C (PP2C) family, plays a crucial role in various cellular processes by regulating protein phosphorylation. Its regulatory function is particularly significant in cell signaling pathways, where it serves to maintain protein homeostasis and modulate responses to stressors. Dysregulation of PPM1B has been implicated in several diseases, including cancer, where it can influence tumor growth and progression by affecting pathways like the MAPK and PI3K/Akt signaling cascades. The study of recombinant PPM1B protein is essential for understanding its biochemical properties, substrate specificity, and interaction with other signaling molecules. Through recombinant expression systems, researchers can produce ample quantities of PPM1B for functional assays, structural analyses, and potential therapeutic targeting. Investigating this protein facilitates the exploration of its role in pathophysiology and may uncover novel strategies for intervention in diseases associated with aberrant phosphatase activity. Overall, PPM1B remains a promising target for both basic research and drug development, emphasizing the need for detailed studies on its functional dynamics in cellular contexts.
