Analytical Data
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Gene name
MDH2
- Application
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Alternative Names
MDH2；Malate dehydrogenase. mitochondrial
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P40926
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Expression Region
25-338aa
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AA Sequence
AKVAVLGASGGIGQPLSLLLKNSPLVSRLTLYDIAHTPGVAADLSHIETKAAVKGYLGPEQLPDCLKGCDVVVIPAGVPRKPGMTRDDLFNTNATIVATLTAACAQHCPEAMICVIANPVNSTIPITAEVFKKHGVYNPNKIFGVTTLDIVRANTFVAELKGLDPARVNVPVIGGHAGKTIIPLISQCTPKVDFPQDQLTALTGRIQEAGTEVVKAKAGAGSATLSMAYAGARFVFSLVDAMNGKEGVVECSFVKSQETECTYFSTPLLLGKKGIEKNLGIGKVSSFEEKMISDAIPELKASIKKGEDFVKTLK
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Molecular Weight
49.0kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
MDH2, or malate dehydrogenase 2, is an important enzyme involved in the tricarboxylic acid (TCA) cycle, catalyzing the interconversion of malate and oxaloacetate while playing a crucial role in cellular metabolism and energy production. As a member of the malate dehydrogenase family, MDH2 primarily functions in the mitochondria, impacting the metabolic pathways that are essential for cellular respiration and the production of ATP. Recent studies have highlighted the significance of MDH2 in various physiological processes and its potential implications in metabolic disorders, cancer, and neurodegenerative diseases. The recombinant expression of MDH2 facilitates detailed biochemical characterization, structure-function studies, and exploration of its interactions with other metabolic enzymes and substrates. By producing MDH2 as a recombinant protein, researchers can obtain large quantities of pure enzyme necessary for kinetic analysis and inhibitor screening, thus understanding its role in health and disease. Additionally, given its involvement in critical metabolic pathways, MDH2 presents a promising target for therapeutic interventions aimed at metabolic regulation. Understanding the structure and function of MDH2 through recombinant protein studies could pave the way for novel strategies in treating metabolic diseases and improving energy homeostasis in cells, making it a significant subject of ongoing biochemical research. As such, MDH2 not only provides insights into fundamental metabolic processes but also represents a key player in the development of innovative treatments for a variety of metabolic disorders.
