Analytical Data
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基因名
POU5F1
- Application
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别名
POU5F1；OCT3；OCT4；OTF3；POU domain. class 5. transcription factor 1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q01860
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表达区间
1-360aa
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氨基酸序列
MAGHLASDFAFSPPPGGGGDGPGGPEPGWVDPRTWLSFQGPPGGPGIGPGVGPGSEVWGIPPCPPPYEFCGGMAYCGPQVGVGLVPQGGLETSQPEGEAGVGVESNSDGASPEPCTVTPGAVKLEKEKLEQNPEESQDIKALQKELEQFAKLLKQKRITLGYTQADVGLTLGVLFGKVFSQTTICRFEALQLSFKNMCKLRPLLQKWVEEADNNENLQEICKAETLVQARKRKRTSIENRVRGNLENLFLQCPKPTLQQISHIAQQLGLEKDVVRVWFCNRRQKGKRSSSDYAQREDFEAAGSPFSGGPVSFPLAPGPHFGTPGYGSPHFTALYSSVPFPEGEAFPPVSVTTLGSPMHSN
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分子量
52.6 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
POU5F1, also known as Oct4, is a critical transcription factor involved in the regulation of pluripotency and self-renewal in embryonic stem cells. Its expression is pivotal for maintaining the undifferentiated state of these cells, and it plays a significant role in early development. Research into POU5F1 has garnered attention for its potential applications in regenerative medicine, particularly in the fields of stem cell therapy and tissue engineering. As a key player in reprogramming somatic cells into induced pluripotent stem cells (iPSCs), POU5F1 serves as a foundational element in the quest to create patient-specific stem cell lines for therapeutic purposes. Studies have shown that the recombinant protein of POU5F1 can effectively modulate gene expression associated with stem cell characteristics, leading to advances in understanding stem cell biology and developmental processes. Furthermore, the investigation of POU5F1 and its interactions with other signaling pathways has important implications for cancer research, as aberrant expression of this factor is often linked to tumorigenesis. Therefore, the development and characterization of recombinant POU5F1 protein provide invaluable insights into both normal physiological processes and disease mechanisms, highlighting its importance as a target for therapeutic intervention.
