Analytical Data
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Gene name
SEMA4A
- Application
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Alternative Names
SEMA4A；SEMAB；SEMB；Semaphorin-4A
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9H3S1
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Expression Region
33-683aa
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AA Sequence
GGGQGPMPRVRYYAGDERRALSFFHQKGLQDFDTLLLSGDGNTLYVGARE AILALDIQDPGVPRLKNMIPWPASDRKKSECAFKKKSNETQCFNFIRVLV SYNVTHLYTCGTFAFSPACTFIELQDSYLLPISEDKVMEGKGQSPFDPAH KHTAVLVDGMLYSGTMNNFLGSEPILMRTLGSQPVLKTDNFLRWLHHDAS FVAAIPSTQVVYFFFEETASEFDFFERLHTSRVARVCKNDVGGEKLLQKK WTTFLKAQLLCTQPGQLPFNVIRHAVLLPADSPTAPHIYAVFTSQWQVGG TRSSAVCAFSLLDIERVFKGKYKELNKETSRWTTYRGPETNPRPGSCSVG PSSDKALTFMKDHFLMDEQVVGTPLLVKSGVEYTRLAVETAQGLDGHSHL VMYLGTTTGSLHKAVVSGDSSAHLVEEIQLFPDPEPVRNLQLAPTQGAVF VGFSGGVWRVPRANCSVYESCVDCVLARDPHCAWDPESRTCCLLSAPNLN SWKQDMERGNPEWACASGPMSRSLRPQSRPQIIKEVLAVPNSILELPCPH LSALASYYWSHGPAAVPEASSTVYNGSLLLIVQDGVGGLYQCWATENGFS YPVISYWVDSQDQTLALDPELAGIPREHVKVPLTRVSGGAALAAQQSYWP H
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Molecular Weight
72 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
SEMA4A, a member of the semaphorin family, plays a pivotal role in various biological processes including neuronal development, immune response, and tumorigenesis. It functions primarily as a signaling molecule, interacting with neuropilin and plexin receptors, thereby influencing cell migration, axon guidance, and modulation of immune cell activities. Recent studies have highlighted the significance of SEMA4A in cancer biology, where its expression levels can correlate with tumor progression and metastasis. Additionally, SEMA4A has been identified as a potential biomarker for several diseases, suggesting its role in disease states beyond the nervous system. The recombinant SEMA4A protein has emerged as a valuable tool for elucidating its biological functions and therapeutic potentials, as it allows researchers to investigate its mechanisms in vitro and in vivo. By exploring the structural and functional attributes of this protein, researchers aim to unveil new pathways and targets for therapeutic intervention, particularly in oncology and autoimmune diseases. Given the evolving landscape of biomedicine, the characterization of recombinant SEMA4A could pave the way for innovative strategies in disease management and treatment.
