Analytical Data
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Gene name
KSR1
- Application
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Alternative Names
KSR1；KSR；Kinase suppressor of Ras 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8IVT5
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Expression Region
335-923aa
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AA Sequence
PQMVRRDIGLSVTHRFSTKSWLSQVCHVCQKSMIFGVKCKHCRLKCHNKC TKEAPACRISFLPLTRLRRTESVPSDINNPVDRAAEPHFGTLPKALTKKE HPPAMNHLDSSSNPSSTTSSTPSSPAPFPTSSNPSSATTPPNPSPGQRDS RFNFPAAYFIHHRQQFIFPVPSAGHCWKCLLIAESLKENAFNISAFAHAA PLPEAADGTRLDDQPKADVLEAHEAEAEEPEAGKSEAEDDEDEVDDLPSS RRPWRGPISRKASQTSVYLQEWDIPFEQVELGEPIGQGRWGRVHRGRWHG EVAIRLLEMDGHNQDHLKLFKKEVMNYRQTRHENVVLFMGACMNPPHLAI ITSFCKGRTLHSFVRDPKTSLDINKTRQIAQEIIKGMGYLHAKGIVHKDL KSKNVFYDNGKVVITDFGLFGISGVVREGRRENQLKLSHDWLCYLAPEIV REMTPGKDEDQLPFSKAADVYAFGTVWYELQARDWPLKNQAAEASIWQIG SGEGMKRVLTSVSLGKEVSEILSACWAFDLQERPSFSLLMDMLEKLPKLN RRLSHPGHFWKSADINSSKVVPRFERFGLGVLESSNPKM
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Molecular Weight
105 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
KSR1 (Kinase Suppressor of Ras 1) is a crucial component of the Ras signaling pathway, which plays a significant role in cell proliferation, differentiation, and survival. Research into KSR1 has gained momentum due to its implications in various cancers, where dysregulation of Ras signaling is often observed. KSR1 functions as a scaffolding protein, facilitating the assembly of different signaling molecules to enhance the efficiency of Ras-mediated signal transduction. It interacts with various kinases, including MEK and ERK, which are vital in the MAPK/ERK pathway. Understanding KSR1's structure and function is essential for elucidating its role in normal cellular processes and its contributions to oncogenesis. The generation of recombinant KSR1 proteins has enabled scientists to study its molecular interactions, post-translational modifications, and functional dynamics in vitro and in vivo. These studies are critical for identifying potential therapeutic targets aimed at modulating KSR1 activity in Ras-driven cancers. Given the complexity of KSR1's role in cell signaling, ongoing research focusing on its mechanisms and effects on cellular pathways holds promise for developing novel cancer treatments and better understanding the intricacies of Ras-dependent signaling networks.
