Analytical Data
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基因名
PIP4K2A
- Application
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别名
PIP4K2A；PIP5K2B；Phosphatidylinositol 5-phosphate 4-kinase type-2 beta
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P48426
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表达区间
2-406aa
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氨基酸序列
ATPGNLGSS VLASKTKTKK KHFVAQKVKL FRASDPLLSV LMWGVNHSIN ELSHVQIPVM LMPDDFKAYS KIKVDNHLFN KENMPSHFKF KEYCPMVFRN LRERFGIDDQ DFQNSLTRSA PLPNDSQARS GARFHTSYDK RYIIKTITSE DVAEMHNILK KYHQYIVECH GITLLPQFLG MYRLNVDGVE IYVIVTRNVF SHRLSVYRKY DLKGSTVARE ASDKEKAKEL PTLKDNDFIN EGQKIYIDDN NKKVFLEKLK KDVEFLAQLK LMDYSLLVGI HDVERAEQEE VECEENDGEE EGESDGTHPV GTPPDSPGNT LNSSPPLAPG EFDPNIDVYG IKCHENSPRK EVYFMAIIDI LTHYDAKKKA AHAAKTVKHG AGAEISTVNP EQYSKRFLDF IGHILT
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PIP4K2A, or Phosphatidylinositol 5-phosphate 4-kinase type 2 alpha, is an important enzyme involved in the synthesis of phosphoinositides, which play crucial roles in cellular signaling, membrane dynamics, and intracellular trafficking. Research into PIP4K2A has gained momentum due to its implications in various physiological processes and diseases, including cancer, neurodegenerative disorders, and metabolic diseases. The enzyme phosphorylates phosphatidylinositol 5-phosphate (PI5P) to produce phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), a vital component of cellular membranes that serves as a precursor for signaling molecules. Studies have shown that PIP4K2A is crucial for maintaining cellular homeostasis and participates in regulating actin cytoskeleton dynamics, thereby influencing cell migration and proliferation. Its dysregulation has been linked to tumorigenesis and has emerged as a potential therapeutic target. The recombinant expression and purification of PIP4K2A allow for detailed biochemical characterization and structural studies, which are essential for understanding its enzymatic mechanisms and interactions with other cellular components. Furthermore, elucidating the role of PIP4K2A in cellular pathways may reveal novel approaches for drug development aimed at modulating its activity in disease contexts. As a result, the research surrounding PIP4K2A not only enhances our understanding of phosphoinositide signaling but also provides a foundation for exploring new therapeutic avenues in various diseases.
