Analytical Data
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Gene name
INPP5A
- Application
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Alternative Names
INPP5A；5PTASE；Inositol polyphosphate-5-phosphatase A
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q14642
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Expression Region
1-410aa
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AA Sequence
MAGKAAAPGTAVLLVTANVGSLFDDPENLQKNWLREFYQVVHTHKPHFMA LHCQEFGGKNYEASMSHVDKFVKELLSSDAMKEYNRARVYLDENYKSQEH FTALGSFYFLHESLKNIYQFDFKAKKYRKVAGKEIYSDTLESTPMLEKEK FPQDYFPECKWSRKGFIRTRWCIADCAFDLVNIHLFHDASNLVAWETSPS VYSGIRHKALGYVLDRIIDQRFEKVSYFVFGDFNFRLDSKSVVETLCTKA TMQTVRAADTNEVVKLIFRESDNDRKVMLQLEKKLFDYFNQEVFRDNNGT ALLEFDKELSVFKDRLYELDISFPPSYPYSEDARQGEQYMNTRCPAWCDR ILMSPSAKELVLRSESEEKVVTYDHIGPNVCMGDHKPVFLAFRIMPGAGK PHAHVHKCCVDHHHHHH
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Molecular Weight
49 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
INPP5A (Inositol Polyphosphate-5-Phosphatase A) is a key enzyme involved in the regulation of several cellular processes, including signal transduction and phosphoinositide metabolism. By hydrolyzing inositol trisphosphate and playing a crucial role in the generation of second messengers, INPP5A influences various physiological functions such as cell growth, differentiation, and apoptosis. Its dysregulation has been implicated in a variety of diseases, including cancer, where altered phosphoinositide signaling contributes to tumorigenesis and metastasis. The study of recombinant INPP5A protein is essential for understanding its biochemical properties, substrate specificity, and interaction with other signaling molecules. These investigations can uncover the pathways that INPP5A modulates and reveal its potential as a therapeutic target. Advances in recombinant DNA technology and protein purification methods have facilitated the expression and characterization of functional INPP5A protein, allowing researchers to explore its role in cellular signaling further. Understanding the structural and functional aspects of INPP5A not only enhances our fundamental knowledge of phosphoinositide metabolism but also paves the way for the development of novel strategies to manipulate its activity in disease contexts.
