Analytical Data
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Gene name
USP5
- Application
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Alternative Names
USP5；ISOT；Ubiquitin carboxyl-terminal hydrolase 5
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P45974
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Expression Region
2-858aa
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AA Sequence
AELSEEALLSVLPTIRVPKAGDRVHKDECAFSFDTPESEGGLYICMNTFL GFGKQYVERHFNKTGQRVYLHLRRTRRPKEEDPATGTGDPPRKKPTRLAI GVEGGFDLSEEKFELDEDVKIVILPDYLEIARDGLGGLPDIVRDRVTSAV EALLSADSASRKQEVQAWDGEVRQVSKHAFSLKQLDNPARIPPCGWKCSK CDMRENLWLNLTDGSILCGRRYFDGSGGNNHAVEHYRETGYPLAVKLGTI TPDGADVYSYDEDDMVLDPSLAEHLSHFGIDMLKMQKTDKTMTELEIDMN QRIGEWELIQESGVPLKPLFGPGYTGIRNLGNSCYLNSVVQVLFSIPDFQ RKYVDKLEKIFQNAPTDPTQDFSTQVAKLGHGLLSGEYSKPVPESGDGER VPEQKEVQDGIAPRMFKALIGKGHPEFSTNRQQDAQEFFLHLINMVERNC RSSENPNEVFRFLVEEKIKCLATEKVKYTQRVDYIMQLPVPMDAALNKEE LLEYEEKKRQAEEEKMALPELVRAQVPFSSCLEAYGAPEQVDDFWSTALQ AKSVAVKTTRFASFPDYLVIQIKKFTFGLDWVPKKLDVSIEMPEELDISQ LRGTGLQPGEEELPDIAPPLVTPDEPKGSLGFYGNEDEDSFCSPHFSSPT SPMLDESVIIQLVEMGFPMDACRKAVYYTGNSGAEAAMNWVMSHMDDPDF ANPLILPGSSGPGSTSAAADPPPEDCVTTIVSMGFSRDQALKALRATNNS LERAVDWIFSHIDDLDAEAAMDISEGRSAADSISESVPVGPKVRDGPGKY QLFAFISHMGTSTMCGHYVCHIKKEGRWVIYNDQKVCASEKPPKDLGYIY FYQRVAS
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Molecular Weight
96 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Ubiquitin-specific protease 5 (USP5) is a member of the deubiquitinating enzymes (DUBs) family, playing a crucial role in the regulation of cellular processes by reversing ubiquitination, a post-translational modification that affects protein stability, localization, and activity. Dysregulation of ubiquitin signaling has been linked to several diseases, including cancer, neurodegenerative disorders, and immune dysfunctions. USP5 is particularly noteworthy due to its unique ability to cleave polyubiquitin chains, thus contributing to the recycling of ubiquitin and the restoration of protein function. Research has shown that USP5 is involved in various biological processes, such as cell proliferation, apoptosis, and DNA repair, highlighting its significance in maintaining cellular homeostasis. Furthermore, the modulation of USP5 activity has emerged as a potential therapeutic strategy in oncology, making it an attractive target for drug development. Understanding the structure, function, and regulatory mechanisms of USP5 through the study of its recombinant protein can provide insights into its specific roles in disease pathways and aid in the identification of novel therapeutic approaches. By investigating USP5 at the molecular level, researchers aim to delineate its interactions with substrate proteins and other components of the ubiquitin-proteasome system, ultimately contributing to a deeper understanding of its functional implications in health and disease.
