Analytical Data
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Gene name
EPHX2
- Application
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Alternative Names
EPHX2；Bifunctional epoxide hydrolase 2
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P34913
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Expression Region
1-555aa
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AA Sequence
MTLRAAVFDLDGVLALPAVFGVLGRTEEALALPRGLLNDAFQKGGPEGATTRLMKGEITLSQWIPLMEENCRKCSETAKVCLPKNFSIKEIFDKAISARKINRPMLQAALMLRKKGFTTAILTNTWLDDRAERDGLAQLMCELKMHFDFLIESCQVGMVKPEPQIYKFLLDTLKASPSEVVFLDDIGANLKPARDLGMVTILVQDTDTALKELEKVTGIQLLNTPAPLPTSCNPSDMSHGYVTVKPRVRLHFVELGSGPAVCLCHGFPESWYSWRYQIPALAQAGYRVLAMDMKGYGESSAPPEIEEYCMEVLCKEMVTFLDKLGLSQAVFIGHDWGGMLVWYMALFYPERVRAVASLNTPFIPANPNMSPLESIKANPVFDYQLYFQEPGVAEAELEQNLSRTFKSLFRASDESVLSMHKVCEAGGLFVNSPEEPSLSRMVTEEEIQFYVQQFKKSGFRGPLNWYRNMERNWKWACKSLGRKILIPALMVTAEKDFVLVPQMSQHMEDWIPHLKRGHIEDCGHWTQMDKPTEVNQILIKWLDSDARNPPVVSKM
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Molecular Weight
78.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
EPHX2 (epoxide hydrolase 2) is an enzyme that plays a crucial role in the metabolism of epoxides, which are reactive intermediates generated during the biotransformation of various xenobiotics and endogenous compounds. This enzyme is involved in detoxifying potentially harmful epoxide compounds, converting them into more water-soluble, less toxic diols. The significance of EPHX2 in drug metabolism and environmental toxicology has garnered increasing attention, as variations in its gene expression and activity can influence individual susceptibility to toxicants and the efficacy of certain pharmacological agents. Furthermore, EPHX2 has been implicated in various pathological conditions, including cancer and cardiovascular diseases, making it a target for therapeutic intervention. Recombinant EPHX2 protein has been developed to facilitate in-depth studies on its enzymatic mechanisms, substrate specificity, and potential role in disease processes. The use of recombinant technology allows for high yields and purification of the enzyme, enabling researchers to probe its functional properties under controlled conditions. Understanding the biochemical characteristics of EPHX2 could provide insights into its role in human health and disease, and pave the way for the development of biomarkers and targeted therapies. Therefore, ongoing research into EPHX2 recombinant protein contributes to a better understanding of its biological functions and potential applications in toxicology and medicine.
