Analytical Data
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基因名
CSRP3
- Application
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别名
CSRP3；CLP；MLP；Cysteine and glycine-rich Protein 3
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P50461
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表达区间
1-194aa
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氨基酸序列
MPNWGGGAKCGACEKTVYHAEEIQCNGRSFHKTCFHCMACRKALDSTTVAAHESEIYCKVCYGRRYGPKGIGYGQGAGCLSTDTGEHLGLQFQQSPKPARSVTTSNPSKFTAKFGESEKCPRCGKSVYAAEKVMGGGKPWHKTCFRCAICGKSLESTNVTDKDGELYCKVCYAKNFGPTGIGFGGLTQQVEKKE
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分子量
37.0 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CSRP3 (Cysteine and glycine-rich protein 3), also known as the muscle LIM protein (MLP), is a key member of the LIM protein family, primarily expressed in striated muscles, including cardiac and skeletal tissues. It plays a vital role in muscle development, maintenance, and repair by regulating gene expression and interacting with cytoskeletal proteins. Abnormalities in CSRP3 expression or function have been linked to various myopathies and cardiomyopathies, making it a significant target for understanding muscle-related diseases. Research on recombinant CSRP3 protein focuses on elucidating its structural and functional properties, exploring its interactions with other proteins, and assessing its role in signaling pathways involved in muscle cell differentiation and hypertrophy. By producing recombinant CSRP3 in suitable expression systems, scientists aim to achieve sufficient yields for biophysical studies, functional assays, and potential therapeutic applications. Understanding CSRP3 better could pave the way for innovative treatments for muscle degenerative diseases and contribute to developing biomarkers for early diagnosis in muscle pathologies. Through these efforts, the investigation of CSRP3 and its recombinant form holds promise for advancing both basic and applied muscle biology research.
