Cat: PA2000-1933

Recombinant Human CNN3 Protein,His

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Analytical Data

  • Gene name

    CNN3

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    CNN3;Calponin-3

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    Q15417

  • Expression Region

    2-329aa

  • AA Sequence

    THFNKGPSYGLSAEVKNKIASKYDHQAEEDLRNWIEEVTGMSIGPNFQLGLKDGIILCELINKLQPGSVKKVNESSLNWPQLENIGNFIKAIQAYGMKPHDIFEANDLFENGNMTQVQTTLVALAGLAKTKGFHTTIDIGVKYAEKQTRRFDEGKLKAGQSVIGLQMGTNKCASQAGMTAYGTRRHLYDPKMQTDKPFDQTTISLQMGTNKGASQAGMLAPGTRRDIYDQKLTLQPVDNSTISLQMGTNKVASQKGMSVYGLGRQVYDPKYCAAPTEPVIHNGSQGTGTNGSEISDSDYQAEYPDEYHGEYQDDYPRDYQYSDQGIDY

  • Molecular Weight

    40.3 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

Quality inspection process

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Protein Description

The study of CNN3 recombinant proteins has emerged as a significant area of research due to the crucial role that the CNN3 gene plays in cellular processes, particularly in muscle development and differentiation. CNN3, or Calponin 3, is a member of the calponin family of actin-binding proteins, which are known for their involvement in the regulation of contraction and structural integrity of smooth muscle cells. Mutations or dysregulation of CNN3 have been implicated in various muscle-related disorders, making it essential to understand its functional mechanisms. The advent of recombinant DNA technology allows for the production of purified CNN3 proteins, enabling researchers to study their biochemical properties and interactions in detail. This research can provide insights into the molecular pathways influenced by CNN3, paving the way for potential therapeutic strategies for muscle diseases. Furthermore, understanding CNN3's interactions with other cytoskeletal components could reveal new aspects of cellular mechanics and tissue organization, ultimately contributing to the broader field of cell biology and biophysics.

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