Analytical Data
-
Gene name
APOBEC3A
- Application
-
Alternative Names
APOBEC3A；DNA dC->dU-editing enzyme APOBEC-3A
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P31941
-
Expression Region
1-199aa
-
AA Sequence
MEASPASGPRHLMDPHIFTSNFNNGIGRHKTYLCYEVERLDNGTSVKMDQHRGFLHNQAKNLLCGFYGRHAELRFLDLVPSLQLDPAQIYRVTWFISWSPCFSWGCAGEVRAFLQENTHVRLRIFAARIYDYDPLYKEALQMLRDAGAQVSIMTYDEFKHCWDTFVDHQGCPFQPWDGLDEHSQALSGRLRAILQNQGN
-
Molecular Weight
24.5 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
APOBEC3A (Apolipoprotein B mRNA Editing Enzyme Catalytic Polypeptide 3A) is a member of the APOBEC family of proteins, which are known for their role in cytidine deamination, a critical process in RNA editing and DNA mutation. Initially identified as an innate immune factor, APOBEC3A plays a significant role in defending against viral infections, particularly by targeting retroviruses and transposable elements. Its enzymatic activity introduces mutations in viral genomes, thereby hindering their replication and contributing to the host's immune response. Beyond its protective role, recent studies have suggested that APOBEC3A may also be involved in various cellular processes, including cancer development, as its activity can lead to mutagenesis in the host genome. This dual functionality has made APOBEC3A a topic of increasing interest in cancer research, where aberrant expression or dysfunction of the protein may contribute to tumorigenesis. Furthermore, its potential as a target for cancer immunotherapy is being explored, given its role in modulating the immune landscape within tumors. Thus, understanding the structure, regulation, and mechanisms of action of APOBEC3A is crucial for leveraging its properties in therapeutic contexts, as well as for elucidating its impact on genome stability and viral pathogenesis. The ongoing research into APOBEC3A encompasses diverse fields, including virology, immunology, and cancer biology, demonstrating its importance as a multifaceted player in both innate immunity and disease progression.
