Analytical Data
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Gene name
C12orf66
- Application
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Alternative Names
C12orf66KICSTOR complex Protein C12orf66
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96MD2
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Expression Region
1-468aa
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AA Sequence
MGESIPLAAPVPVEQAVLETFFSHLGIFSYDKAKDNVEKEREADKSAGGSWLSLLAALAHLAAAEKVYHSLTYLGQKLGGQSFFSRKDSIRTIYTSLHNELKKVVTGRGALGGTAPHVEELLSHLSEQLCFFVQARMEIADFYEKMYTLSTQKFINAEELVGLLDAIMKKYSSRFHHPILSPLESSFQLEVDVLCHLLKAQAQVSEWKFLPSLVNLHSAHTKLQTWGQIFEKQRETKKHLFGGQSQKAVQPPHLFLWLMKLKNMLLAKFSFYFHEALSRQTTASEMKTLTAKANPDFFGKISSFIRKYDAANVSLIFDNRGSESSQGHGYHHPHSYREAPKGVDQYPAVVSLPSDRPVMHWPNVIMIMTDRTSDLNSLEKVVHFYDDKVQSTYFLTRPEPHFTIVIIFESKKSERDSHFISFLNEVSLALKNPKVFASLKPGSKGSCYQCNYFVAFCLDYSKLLLQRK
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Molecular Weight
79.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
C12orf66, also known as "chromosome 12 open reading frame 66," is a gene that has garnered interest in the field of molecular biology due to its potential implications in various biological processes and diseases. Initial studies have suggested that C12orf66 may play a role in cellular functions such as proliferation, differentiation, and apoptosis, although its specific biological functions remain largely uncharacterized. Recent genomic analyses have linked variations in the C12orf66 gene to several neurological disorders, hinting at its possible role in brain development and function. The protein product of C12orf66 has been reported to interact with other proteins, suggesting its involvement in complex cellular pathways. To further elucidate its function, researchers are focusing on the production and characterization of C12orf66 recombinant protein. This study aims to utilize techniques such as gene cloning, expression in suitable host systems (like E. coli or insect cells), and subsequent purification processes to obtain a functional form of the protein. Understanding the structure and function of C12orf66 recombinant protein may provide insights into its role in health and disease, paving the way for potential therapeutic targets. By revealing the molecular mechanisms via which C12orf66 operates, this research will contribute to the broader knowledge of gene functions linked to neurological health and cellular regulation, ultimately aiming to address unmet medical needs associated with C12orf66-related conditions.
