Cat: PA2000-5940

Recombinant Human C12orf50 Protein,GST

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Analytical Data

  • Gene name

    C12orf50

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    C12orf50Uncharacterized Protein C12orf50

  • Species

    Human

  • Source

    E. coli

  • Tag

    GST-tag at N-terminal

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    Q8NA57

  • Expression Region

    1-414aa

  • AA Sequence

    MEMQQNCSIS CFWETQPLGC VKISCIFYHS KPRNINGLFL PPSSNITLQK EIQEGIPLQS QSQEPLKPQE NISRPIHHPL VLKTNFEEEE EVDEQNDASS LWTKTPEEIE EKRAIKEMCY KSGEYYRFHT PPDILSSKSM TPTAEKQLEK PLENGSELQE GDSLTVPTKL SQYERQGEIK TSLHGKPKTD IAAFENGGGD CYVPQRVIFL GVDESEALTE EKEITISKCS NTKDNKDSPH PKHSLTTRLV PTTHVLNATE NISMKCREDP SSMNDVQPVK KPHFKGVKKR KWIYDEPQNF PNSGMQRAVQ APRPQNKMSY HRNNKNRNAE NASYIHVQRD AVRTVALNAP SRSRPTHGSY NKVHANREPK PNLSPDKYTS TSYNDSAWRK RIPFSKTYSK SEKIYPEPRR NGSK

  • Molecular Weight

    73.7 KDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

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Protein Description

C12orf50, also known as Chromosome 12 Open Reading Frame 50, is a protein encoding gene located on human chromosome 12, and recent studies have highlighted its potential roles in cellular processes and disease mechanisms, particularly in neurodegenerative disorders and certain cancers. Characterized by its unique structural features, C12orf50 exhibits a domain architecture that suggests involvement in protein-protein interactions and cellular signaling pathways. Research has indicated that dysregulation of C12orf50 expression may contribute to pathophysiological conditions, prompting investigations into its functional roles. Additionally, the recombinant production of C12orf50 protein allows for detailed biochemical characterization, facilitating studies into its structure-function relationships and interaction with other molecular partners. Current advancements in recombinant DNA technology, along with high-throughput techniques, have enabled scientists to express and purify C12orf50 in various systems, including bacteria and mammalian cells. This opens avenues for understanding its biological functions through in vitro assays and structural analysis, ultimately contributing to the development of potential therapeutic strategies targeting diseases linked to C12orf50 dysregulation. Researchers are increasingly focused on elucidating the precise mechanisms through which C12orf50 exerts its effects at the molecular level, fostering interest in its role as a biomarker or therapeutic target in relevant disease contexts. Consequently, ongoing studies aim to provide a comprehensive understanding of C12orf50’s biological relevance and its potential implications in human health and disease.

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