Analytical Data
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基因名
btuC
- Application
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别名
btuC；Vitamin B12 import system permease Protein BtuC
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
B0R5G3
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表达区间
1-369aa
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氨基酸序列
MREASARAVAWSAAAGVLLVAVLLVSATIGPEPITLRTVAMAALTELAVPVGASVTMHTHAVPVVSGGLPWPALTIAYAAPLQFGVPETAQVIVGTIRLPRIVLGATVGASLAISGAVLQGFFRNPMADPSIVGVSSGAAVGAVAAITLPSVVVIGVQPAAFAGALIAAFTVYAIATKNGHTPTATLLLSGVAVQTLLGAVTSFLVVNSGREIRPAMYWLMGTLHGSRWHDVEAALPVVVVGSAVLLAYAREMNVLLAGEEDAHTLGVDVDRTKRLLLAVASVVTAAAVSFAGAIGFVGLIVPHAVRLVVGPDHRVLLPVSALTGGAFLVAADTVARATATEPPVGIITALIGAPFFLYLLRDREVRAL
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分子量
40.3 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
btuC, a gene located in the btuB-btuC operon, encodes a protein that plays a crucial role in the transport of cobalamin (vitamin B12) across the bacterial membrane. Research into btuC and its encoded protein is important as cobalamin is an essential cofactor for various enzymatic reactions, and its acquisition is vital for bacterial growth and metabolism. Understanding the structure and function of the btuC protein could provide insights into the mechanisms of cobalamin transport and its regulation. Additionally, the btuC protein has garnered interest due to its potential implications in microbiology and biotechnology, including its role in bacterial pathogenesis and the development of antimicrobial agents. Investigating btuC could lead to the discovery of novel therapeutic strategies to combat bacterial infections, as targeting cobalamin transport could disrupt bacterial growth. Overall, the study of btuC recombinant protein is positioned at the intersection of microbiology, biochemistry, and drug development, highlighting its importance in both basic research and applied sciences.
