Analytical Data
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Gene name
btuC
- Application
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Alternative Names
btuC；Vitamin B12 import system permease Protein BtuC
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
B0R5G3
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Expression Region
1-369aa
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AA Sequence
MREASARAVAWSAAAGVLLVAVLLVSATIGPEPITLRTVAMAALTELAVPVGASVTMHTHAVPVVSGGLPWPALTIAYAAPLQFGVPETAQVIVGTIRLPRIVLGATVGASLAISGAVLQGFFRNPMADPSIVGVSSGAAVGAVAAITLPSVVVIGVQPAAFAGALIAAFTVYAIATKNGHTPTATLLLSGVAVQTLLGAVTSFLVVNSGREIRPAMYWLMGTLHGSRWHDVEAALPVVVVGSAVLLAYAREMNVLLAGEEDAHTLGVDVDRTKRLLLAVASVVTAAAVSFAGAIGFVGLIVPHAVRLVVGPDHRVLLPVSALTGGAFLVAADTVARATATEPPVGIITALIGAPFFLYLLRDREVRAL
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Molecular Weight
40.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
btuC, a gene located in the btuB-btuC operon, encodes a protein that plays a crucial role in the transport of cobalamin (vitamin B12) across the bacterial membrane. Research into btuC and its encoded protein is important as cobalamin is an essential cofactor for various enzymatic reactions, and its acquisition is vital for bacterial growth and metabolism. Understanding the structure and function of the btuC protein could provide insights into the mechanisms of cobalamin transport and its regulation. Additionally, the btuC protein has garnered interest due to its potential implications in microbiology and biotechnology, including its role in bacterial pathogenesis and the development of antimicrobial agents. Investigating btuC could lead to the discovery of novel therapeutic strategies to combat bacterial infections, as targeting cobalamin transport could disrupt bacterial growth. Overall, the study of btuC recombinant protein is positioned at the intersection of microbiology, biochemistry, and drug development, highlighting its importance in both basic research and applied sciences.
