Analytical Data
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基因名
ORF1a
- Application
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别名
ORF1a；
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种属
E.coli
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q00020
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表达区间
1-966aa
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氨基酸序列
MSSFVNLESLISERGANCRGADEIVNNETTRILTSQIEHSQRSKKVNIRNKLSVAECDAFRARYGGAFDVNLTHEYTAPHSLAGALRVAEHYDCIDSFPPEDKIIDFGGSWLHHYSRGDSRVHSCCPILGPRDATRHEERMCRLRKMVQTSDRFVDVPDFCLNKAEDCNVQADWAICIHGGYDMGFQGLCKAMHAPLERGILQGTIMFDGAMLFDRQGELPLLQCRWQRVGTGSKEQIKFDFINESTLSYVHDWKNLGSFLTESTYSIGGTTYLLERMLLKCSIMTYKIIATNVRCPPESLRHCIWFENISQYLAVQIPIGYNLNDWKTVRVARATVREVEEISFRCFKENKDWTENMRSVASILSAKSSTVIINGQSIMSGERLDVLEYHLVAFSLTLNLYQKYEKLRNFQGELEWKGWANHFKTRLWWCGRTVSTEGGFLRNFLADKIPWLKLNTYADSLDFITKISEVESFEVDSVPTSRLRSFFQKEENIVERAASEIMSANARRIAKKAEMSKEFDDFVDAPEEFAPEDVVEEVINTPVTQDVKLRQSKPETARSIVLDPDAVLKNGAINEFADYSKRLHENTVSNLRHLWTLMGCRGNEIHNKSVAETYHRVDDMVNVHFPNGHWMYPLKYEYTVGYNDGGLGEKFENELYVVDKTCSCANAKAIADACKKVSAPTCSVVMVDGVAGCGKTTAIKETFRFEKDIIVTANRKSAEDVRKAIFGDASDSEVALKVVRTADSAIMHGLPECHRLLVDEAGLLHYGQLLAVADLCKCSEVLAFGDTEQISFKSRDATFRMKYCNIEYDKRDIVSKTFRCPQDVVSAVKILKRKCANRSSKYNGWVSSSKVEKSLSKSRIVSINQVSMEKHKFYLTMTEADKAALCSRAKDVGLDKTWVESNMETVHEAQGKAVDHVVLVRLKSTKCDLFKSEEYCLVALTRHKRTFEYLYNGDLGGDLISFYVT
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分子量
109 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The ORF1a protein, derived from the open reading frame 1a of coronaviruses, plays a crucial role in the viral life cycle, particularly in the replication and transcription processes. As the first segment of the viral genome, ORF1a encodes several non-structural proteins (nsps), which are essential for the formation of the replication-transcription complex (RTC) and inhibit host immune responses. The study of ORF1a has gained significant attention due to its implications in understanding coronavirus pathogenesis and its potential as a target for therapeutic interventions and vaccine development. The ongoing research is particularly relevant in the context of emerging viral variants and the global response to pandemics, including the COVID-19 outbreak caused by SARS-CoV-2. Recent studies have focused on characterizing the biochemical properties of ORF1a, including its interactions with host cell factors and its enzymatic activities, aiming to unravel the intricate molecular mechanisms that facilitate viral replication and immune evasion. Furthermore, the exploration of ORF1a-derived proteins as potential biomarkers or targets for drug development underscores the importance of this area of research in addressing public health challenges posed by coronaviruses. As new variants continue to emerge, understanding the role of ORF1a in viral fitness and pathogenicity is essential for devising effective strategies to combat coronavirus infections.
