Analytical Data
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Gene name
PPFIA4
- Application
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Alternative Names
KIAA0897; LIPA4_HUMAN; Liprin alpha 4; Liprin-alpha-4; PPFIA 4; PPFIA4; Protein tyrosine phosphatase receptor type f polypeptide (PTPRF) interacting protein (liprin) alpha 4; Protein tyrosine phosphatase receptor type f polypeptide interacting protein alpha 4; Protein tyrosine phosphatase receptor type f polypeptide-interacting protein alpha-4; PTPRF interacting protein alpha 4; PTPRF-interacting protein alpha-4
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O75335
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Expression Region
1-701 aa
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AA Sequence
MGSAADVRFS LGTTTHAPPG VHRRYSALRE ESAKDWETSP LPGMLAPAAG PAFDSDPEIS DVDEDEPGGL VGSADVVSPS GHSDAQTLAM MLQEQLDAIN EEIRMIQEEK ESTELRAEEI ETRVTSGSME ALNLKQLRKR GSIPTSLTAL SLASASPPLS GRSTPKLTSR SAAQDLDRMG VMTLPSDLRK HRRKLLSPVS REENREDKAT IKCETSPPSS PRTLRLEKLG HPALSQEEGK SALEDQGSNP SSSNSSQDSL HKGAKRKGIK SSIGRLFGKK EKGRLIQLSR DGATGHVLLT DSEFSMQEPM VPAKLGTQAE KDRRLKKKHQ LLEDARRKGM PFAQWDGPTV VSWLELWVGM PAWYVAACRA NVKSGAIMSA LSDTEIQREI GISNALHRLK LRLAIQEMVS LTSPSAPPTS RTSSGNVWVT HEEMETLETS TKTDSEEGSW AQTLAYGDMN HEWIGNEWLP SLGLPQYRSY FMECLVDARM LDHLTKKDLR VHLKMVDSFH RTSLQYGIMC LKRLNYDRKE LEKRREESQH EIKDVLVWTN DQVVHWVQSI GLRDYAGNLH ESGVHGALLA LDENFDHNTL ALILQIPTQN TQARQVMERE FNNLLALGTD RKLDDGDDKV FRRAPSWRKR FRPREHHGRG GMLSASAETL PAGFRVSTLG TLQPPPAPPK KIMPEAHSHY LYGHMLSAFR D
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Molecular Weight
134.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PPFIA4, also known as Protein Phosphatase 1, regulatory subunit 14, plays a crucial role in various cellular processes, including signal transduction, neuronal development, and synaptic function. It is part of the protein phosphatase 1 (PP1) family, which is integral to the regulation of numerous physiological pathways by dephosphorylating serine and threonine residues on target proteins. Research on PPFIA4 has garnered attention due to its implications in neurobiology, particularly in relation to cognitive functions and synaptic plasticity, which are fundamental for learning and memory. Additionally, emerging studies indicate that alterations in PPFIA4 expression or function may be linked to several neurological disorders, including Alzheimer's disease and schizophrenia. The exploration of PPFIA4 as a potential therapeutic target has led to increased interest in its structural characteristics, interaction partners, and regulatory mechanisms. Understanding the biochemical properties and functional roles of the PPFIA4 recombinant protein is essential for delineating its contribution to cellular signaling networks and its potential role in disease mechanisms. This research could pave the way for novel therapeutic approaches aimed at modulating PP1 activity in the context of neurodegenerative diseases and other disorders associated with dysregulated phosphorylation processes.
