Analytical Data
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基因名
PORCN
- Application
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别名
Protein MG61
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9H237
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表达区间
1-461 aa
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氨基酸序列
MATFSRQEFFQQLLQGCLLPTAQQGLDQIWLLLAICLACRLLWRLGLPSYLKHASTVAGGFFSLYHFFQLHMVWVVLLSLLCYLVLFLCRHSSHRGVFLSVTILIYLLMGEMHMVDTVTWHKMRGAQMIVAMKAVSLGFDLDRGEVGTVPSPVEFMGYLYFVGTIVFGPWISFHSYLQAVQGRPLSCRWLQKVARSLALALLCLVLSTCVGPYLFPYFIPLNGDRLLRNKKRKARGTMVRWLRAYESAVSFHFSNYFVGFLSEATATLAGAGFTEEKDHLEWDLTVSKPLNVELPRSMVEVVTSWNLPMSYWLNNYVFKNALRLGTFSAVLVTYAASALLHGFSFHLAAVLLSLAFITYVEHVLRKRLARILSACVLSKRCPPDCSHQHRLGLGVRALNLLFGALAIFHLAYLGSLFDVDVDDTTEEQGYGMAYTVHKWSELSWASHWVTFGCWIFYRLIG
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分子量
53.8 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PORCN (porcupine O-acyltransferase) is a critical enzyme involved in the Wnt signaling pathway, a key regulator of cell growth, differentiation, and tissue homeostasis. Dysregulation of this pathway has been implicated in various diseases, including cancer, neurodegenerative disorders, and developmental anomalies. PORCN catalyzes the palmitoylation of Wnt ligands, a modification necessary for their secretion and signaling capacity. Due to its pivotal role, PORCN has emerged as an attractive therapeutic target for the development of small-molecule inhibitors aimed at modulating Wnt signaling in pathological conditions. Research into PORCN has expanded our understanding of Wnt biology and its influence on stem cell behavior and tumorigenesis, highlighting the enzyme's potential in both basic and translational research. The ongoing study of PORCN-recombinant proteins aims to elucidate the finer mechanistic details of palmitoylation and its effects on the Wnt signaling cascade. These efforts provide a foundation for innovative treatment strategies targeting PORCN-related pathways, potentially leading to novel interventions for diseases driven by aberrant Wnt signaling.
