Analytical Data
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基因名
POLRMT
- Application
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别名
APOLMT; DNA-directed RNA polymerase; DNA-directed RNA polymerase mitochondrial; h-mtRPOL; mitochondrial; MTRNAP; MtRPOL; POLRMT; polymerase (RNA) mitochondrial (DNA directed); polymerase. RNA. mitochondrial; RPOM; RPOM_HUMAN
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O00411
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表达区间
1121-1230 aa
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氨基酸序列
PNFIHSLDSSHMMLTALHCYRKGLTFVSVHDCYWTHAADVSVMNQVCREQFVRLHSEPILQDLSRFLVKRFCSEPQKILEASQLKETLQAVPKPGAFDLEQVKRSTYFFS
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分子量
37.84 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
POLRMT, or mitochondrial RNA polymerase, is a crucial enzyme responsible for the transcription of mitochondrial DNA (mtDNA), which encodes essential proteins for mitochondrial function and energy production. The study of POLRMT has gained significant attention due to its pivotal role in cellular metabolism and its implications in various human diseases, including mitochondrial disorders and certain cancers. Research has shown that mutations in POLRMT can lead to impaired mitochondrial function, resulting in a range of metabolic deficiencies and contributing to the progression of age-related diseases. Additionally, understanding POLRMT's structure and function can provide insights into the regulation of mtDNA transcription and its interaction with other mitochondrial proteins. Recent advancements in recombinant protein technology have facilitated the production of POLRMT in vitro, allowing for detailed biochemical analyses and the exploration of its mechanisms of action. This research not only aims to elucidate the fundamental biological processes governing mitochondrial gene expression but also seeks to identify potential therapeutic targets for treating mitochondrial dysfunction-related diseases. As such, POLRMT stands at the intersection of basic research and clinical applications, driving a deeper understanding of mitochondrial biology and its broader implications for human health.
