Analytical Data
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基因名
POLR3H
- Application
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别名
POLR3H; KIAA1665; RPC8; DNA-directed RNA polymerase III subunit RPC8; RNA polymerase III subunit C8; DNA-directed RNA polymerase III subunit H; RNA polymerase III subunit 22.9 kDa subunit; RPC22.9
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9Y535
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表达区间
1-204 aa
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氨基酸序列
MFVLVEMVDT VRIPPWQFER KLNDSIAEEL NKKLANKVVY NVGLCICLFD ITKLEDAYVF PGDGASHTKV HFRCVVFHPF LDEILIGKIK GCSPEGVHVS LGFFDDILIP PESLQQPAKF DEAEQVWVWE YETEEGAHDL YMDTGEEIRF RVVDESFVDT SPTGPSSADA TTSSEELPKK EAPYTLVGSI SEPGLGLLSW WTSN
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分子量
22.9 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
POLR3H, a key component of the RNA polymerase III complex, plays a critical role in the transcription of small non-coding RNAs, such as 5S rRNA and tRNA. Understanding its function is essential for elucidating the mechanisms of gene expression regulation and cellular stress responses. Research into POLR3H has gained momentum due to its involvement in various biological processes and potential links to disease states, including cancer and genetic disorders. Recent studies have highlighted that alterations in POLR3H expression can affect the overall activity of RNA polymerase III, leading to dysregulation of essential molecular pathways. Moreover, its role in the maturation and processing of certain RNA molecules emphasizes its importance in maintaining cellular homeostasis. As such, recombinant POLR3H protein has emerged as a valuable tool for biophysical and biochemical studies that aim to define the precise molecular interactions and functional dynamics of the RNA polymerase III machinery. By generating and characterizing this recombinant protein, researchers aim to better understand its structure-function relationships, the effects of post-translational modifications, and its potential as a therapeutic target in diseases associated with transcriptional dysregulation. Overall, the study of POLR3H and its recombinant form represents a critical avenue for advancing our understanding of gene regulation and developing novel interventions for related pathologies.
