Analytical Data
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Gene name
POLR3G
- Application
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Alternative Names
DNA directed RNA polymerase III 32 kDa polypeptide; DNA-directed RNA polymerase III subunit G; DNA-directed RNA polymerase III subunit RPC7; Polr3g; Polymerase (RNA) III (DNA directed) polypeptide G (32kD); RNA polymerase III 32 kDa subunit; RNA polymerase III subunit C7; RPC32; RPC7; RPC7_HUMAN
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O15318
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Expression Region
1-223 aa
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AA Sequence
MAGNKGRGRA AYTFNIEAVG FSKGEKLPDV VLKPPPLFPD TDYKPVPLKT GEGEEYMLAL KQELRETMKR MPYFIETPEE RQDIERYSKR YMKVYKEEWI PDWRRLPREM MPRNKCKKAG PKPKKAKDAG KGTPLTNTED VLKKMEELEK RGDGEKSDEE NEEKEGSKEK SKEGDDDDDD DAAEQEEYDE EEQEEENDYI NSYFEDGDDF GADSDDNMDE ATY
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Molecular Weight
25.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
POLR3G (RNA polymerase III subunit G) is a crucial component of RNA polymerase III, which is responsible for synthesizing small RNAs, such as tRNA and 5S rRNA, that play essential roles in various cellular processes. Recent studies have highlighted its significance not only in transcription but also in regulating gene expression and maintaining genomic stability. Aberrations in POLR3G expression have been associated with various diseases, including cancer and neurodegenerative disorders. The investigation of POLR3G recombinant proteins has gained momentum as researchers aim to elucidate the molecular mechanisms underlying its function. By producing POLR3G in a recombinant system, scientists can analyze its structure, interaction with other protein components, and its role in the transcriptional machinery. Understanding these aspects could provide insights into the pathophysiology of diseases linked to POLR3G dysfunction and open new avenues for therapeutic intervention. Additionally, recombinant POLR3G can serve as a valuable tool in biotechnological applications, such as synthetic biology and RNA-based therapies, making its study a promising frontier in molecular biology and medicine.
