Analytical Data
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Gene name
POLK
- Application
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Alternative Names
DinB homolog 1 (E. coli); DINB protein; DINB1; DINP; DNA damage inducible protein b; DNA polymerase kappa; Polk; POLK_HUMAN; POLQ; Polymerase (DNA directed) kappa; polymerase. DNA. kappa
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9UBT6
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Expression Region
1-472 aa
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AA Sequence
MDSTKEKCDSYKDDLLLRMGLNDNKAGMEGLDKEKINKIIMEATKGSRFYGNELKKEKQVNQRIENMMQQKAQITSQQLRKAQLQVDRFAMELEQSRNLSNTIVHIDMDAFYAAVEMRDNPELKDKPIAVGSMSMLSTSNYHARRFGVRAAMPGFIAKRLCPQLIIVPPNFDKYRAVSKEVKEILADYDPNFMAMSLDEAYLNITKHLEERQNWPEDKRRYFIKMGSSVENDNPGKEVNKLSEHERSISPLLFEESPSDVQPPGDPFQVNFEEQNNPQILQNSVVFGTSAQEVVKEIRFRIEQKTTLTASAGIAPNTMLAKVCSDKNKPNGQYQILPNRQAVMDFIKDLPIRKVSGIGKVTEKMLKALGIITCTELYQQRALLSLLFSETSWHYFLHISLGLGSTHLTRDGERKSMSVERTFSEINKAEEQYSLCQELCSELAQDLQKERLKVLYFDMVSLVFKFFNSKMLP
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Molecular Weight
77.66 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
POLK (Polymerase Kappa) is a member of the Y-family DNA polymerases, which are crucial for DNA repair and replication under stress conditions, such as during the bypass of DNA lesions. Studies have shown that POLK has unique properties that enable it to accurately incorporate nucleotides opposite damaged bases, which is essential for maintaining genomic stability. Its roles in translesion synthesis (TLS) have garnered significant attention, as incorrect replication can lead to mutations and contribute to diseases, including cancer. Moreover, POLK's involvement in various cellular processes, including cell cycle regulation and apoptosis, highlights its importance beyond DNA repair. With advancements in structural biology and biochemistry, researchers aim to elucidate POLK’s mechanisms of action, regulation, and interaction with other proteins involved in DNA metabolism. Understanding POLK could lead to novel therapeutic approaches, particularly in cancers where TLS pathways are dysregulated. As a target for drug development, detailed knowledge of POLK’s structure and function will be vital in designing interventions that could enhance genomic fidelity and combat cancer progression. Thus, POLK is a significant focus in current molecular biology research, providing insights into the fundamental processes of DNA damage response and repair.
