Analytical Data
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基因名
PLEKHM2
- Application
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别名
PLEKHM2; KIAA0842; SKIP; Pleckstrin homology domain-containing family M member 2; PH domain-containing family M member 2; Salmonella-induced filaments A and kinesin-interacting protein; SifA and kinesin-interacting protein
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q8IWE5
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表达区间
1-440 aa
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氨基酸序列
MVHSSEFRVDNNHLLLLMIHVFRENEEQLFKMIRMSTGHMEGNLQLLYVLLTDCYVYLLRKGATEKPYLVEEAVSYNELDYVSVGLDQQTVKLVCTNRRKQFLLDTADVALAEFFLASLKSAMIKGCREPPYPSILTDATMEKLALAKFVAQESKCEASAVTVRFYGLVHWEDPTDESLGPTPCHCSPPEGTITKEGMLHYKAGTSYLGKEHWKTCFVVLSNGILYQYPDRTDVIPLLSVNMGGEQCGGCRRANTTDRPHAFQVILSDRPCLELSAESEAEMAEWMQHLCQAVSKGVIPQGVAPSPCIPCCLVLTDDRLFTCHEDCQTSFFRSLGTAKLGDISAVSTEPGKEYCVLEFSQDSQQLLPPWVIYLSCTSELDRLLSALNSGWKTIYQVDLPHTAIQEASNKKKFEDALSLIHSAWQRSDSLCRGRASRDPWC
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分子量
75.8 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PLEKHM2 (pleckstrin homology domain containing M2) is a protein that has garnered attention in recent years due to its involvement in various cellular processes, particularly in endosomal and lysosomal trafficking. Its role in intracellular signaling and potential links to diseases, including cancer and neurodegeneration, make it a compelling subject for study. Researchers have identified PLEKHM2 as a key regulator of autophagy, a catabolic process essential for maintaining cellular homeostasis. The protein interacts with multiple membrane-bound organelles, suggesting it may play a critical role in the transport and degradation of cellular components. Additionally, its pleckstrin homology domain is thought to facilitate the binding of PLEKHM2 to phosphoinositides, which are vital for membrane dynamics. Recombinant expression of PLEKHM2 is crucial for further elucidating its biochemical characteristics and functional mechanisms. By generating PLEKHM2 recombinant proteins, scientists aim to explore its interactions, post-translational modifications, and involvement in signaling pathways. Such studies could provide insights into the protein's structure-function relationship and its potential as a therapeutic target. Understanding PLEKHM2’s role in cellular processes not only enhances our knowledge of basic biological mechanisms but also opens avenues for developing novel strategies in treating diseases linked to dysregulated autophagy and intracellular trafficking. As research progresses, the exploration of PLEKHM2's implications in health and disease highlights the importance of this protein in understanding complex cellular functions and its potential applicability in biomedicine.
