Analytical Data
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Gene name
PIWIL4
- Application
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Alternative Names
DKFZp686P01248; FLJ36156; HILI 2; HILI2 ; HIWI 2; HIWI2; Miwi 2 protein; Miwi2; PIWI; Piwi like 2; Piwi like 4 (Drosophila) ; Piwi like 4; Piwi like protein 4; PIWI like protein; Piwi like RNA mediated gene silencing 4; Piwi-like protein 4; PIWIL 4; Piwil4; PIWL4_HUMAN
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q7Z3Z4
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Expression Region
1-852 aa
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AA Sequence
MSGRARVKARGIARSPSATEVGRIQASPLPRSVDLSNNEASSSNGFLGTSRISTNDKYGISSGDAGSTFMERGVKNKQDFMDLSICTREKLAHVRNCKTGSSGIPVKLVTNLFNLDFPQDWQLYQYHVTYIPDLASRRLRIALLYSHSELSNKAKAFDGAILFLSQKLEEKVTELSSETQRGETIKMTITLKRELPSSSPVCIQVFNIIFRKILKKLSMYQIGRNFYNPSEPMEIPQHKLSLWPGFAISVSYFERKLLFSADVSYKVLRNETVLEFMTALCQRTGLSCFTQTCEKQLIGLIVLTRYNNRTYSIDDIDWSVKPTHTFQKRDGTEITYVDYYKQQYDITVSDLNQPMLVSLLKKKRNDNSEAQLAHLIPELCFLTGLTDQATSDFQLMKAVAEKTRLSPSGRQQRLARLVDNIQRNTNARFELETWGLHFGSQISLTGRIVPSEKILMQDHICQPVSAADWSKDIRTCKILNAQSLNTWLILCSDRTEYVAESFLNCLRRVAGSMGFNVDYPKIIKVQENPAAFVRAIQQYVDPDVQLVMCILPSNQKTYYDSIKKYLSSDCPVPSQCVLARTLNKQGMMMSIATKIAMQMTCKLGGELWAVEIPLKSLMVVGIDVCKDALSKDVMVVGCVASVNPRITRWFSRCILQRTMTDVADCLKVFMTGALNKWYKYNHDLPARIIVYRAGVGDGQLKTLIEYEVPQLLSSVAESSSNTSSRLSVIVVRKKCMPRFFTEMNRTVQNPPLGTVVDSEATRNEWYDFYLISQVACRGTVSPTYYNVIYDDNGLKPDHMQRLTFKLCHLYYNWPGIVSVPAPCQYAHKLTFLVAQSIHKEPSLELANHLFYL
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Molecular Weight
120.12 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PIWIL4, a member of the Argonaute protein family, plays a crucial role in RNA silencing and regulation of gene expression, particularly in the context of germline development and stem cell maintenance. Research on PIWIL4 has gained attention due to its involvement in the regulation of transposable elements and its potential implications in tumorigenesis, as aberrant expression of piRNAs (PIWI-interacting RNAs) associated with PIWIL4 is linked to various cancers. Understanding the structural and functional properties of PIWIL4 is essential for deciphering its mechanisms in cellular processes. The recombinant protein of PIWIL4 allows researchers to investigate its biochemical properties, interactions with piRNAs, and the pathways it influences at a molecular level. This research not only contributes to the basic understanding of gene regulation but may also pave the way for new therapeutic approaches targeting PIWIL4-related pathways in cancer treatment and fertility preservation. The study of this protein, therefore, holds promise for advancing both basic science and clinical applications.
