Analytical Data
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Gene name
BBS5
- Application
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Alternative Names
Bardet Biedl syndrome 5 Protein; Bardet-Biedl syndrome 5 Protein; BBS5; BBS5_HUMAN
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8N3I7
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Expression Region
1-341aa
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AA Sequence
MSVLDALWEDRDVRFDLSAQQMKTRPGEVLIDCLDSIEDTKGNNGDRGRLLVTNLRILWHSLALSRVNVSVGYNCILNITTRTANSKLRGQTEALYILTKCNSTRFEFIFTNLVPGSPRLFTSVMAVHRAYETSKMYRDFKLRSALIQNKQLRLLPQEHVYDKINGVWNLSSDQGNLGTFFITNVRIVWHANMNDSFNVSIPYLQIRSIKIRDSKFGLALVIESSQQSGGYVLGFKIDPVEKLQESVKEINSLHKVYSASPIFGVDYEMEEKPQPLEALTVEQIQDDVEIDSDGHTDAFVAYFADGNKQQDREPVFSEELGLAIEKLKDGFTLQGLWEVMS
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Molecular Weight
65.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
BBS5, or Bardet-Biedl syndrome 5, is a protein associated with Bardet-Biedl Syndrome (BBS), a genetically heterogeneous disorder characterized by multiple phenotypic features, including obesity, retinal degeneration, polydactyly, and kidney abnormalities. Research into BBS5 has gained prominence due to its role in ciliary function and cellular signaling pathways. Cilia are essential cellular structures involved in various signaling mechanisms, and mutations in BBS-associated genes disrupt normal ciliary function, leading to the diverse symptoms observed in BBS patients. BBS5 is part of the BBSome complex, which plays a critical role in the transport of proteins and lipids to the cilia. Understanding the structure and function of BBS5 is vital for elucidating the molecular mechanisms underlying BBS and can shed light on related ciliopathies. Recent studies have focused on recombinant protein expression, purification, and functional assays to characterize BBS5 and its interactions with other components of the BBSome. These investigations are crucial for developing potential therapeutic strategies and better understanding the broader implications of BBS5 functions in cellular health and disease.
