Analytical Data
-
基因名
PIP5K1A
- Application
-
别名
PIPSL; PSMD4P2; Putative PIP5K1A and PSMD4-like protein; PIP5K1A-PSMD4
-
种属
Human
-
表达系统
E. coli
-
标签
GST-tag at N-terminal
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
A2A3N6
-
表达区间
1-500 aa
-
氨基酸序列
MASASSGPSSSVGFSSFDPAVPSCTLSSASGIKRPMASEVPYASGMPIKKIGHRSVDSSGETTYKKTTSSALKGAIQLGITHTVGSLSTKPERDVLMQDFYVVESIFFPSEGSNLTPAHHYNDFRFKTYAPVAFRYFRELFGIRPDDYLYSLCSEPLIELCSSGASGSLFYVSSDDEFIIKTVQHKEAEFLQKLLPGYYMNLNQNPRTLLPKFYGLYCVQAGGKNIRIVVMNNLLPRSVKMHIKYDLKGSTYKRRASQKEREKPLPTFKDLDFLQDIPDGLFLDADMYNALCKTLQRDCLVLQSFKIMDYSLLMSIHNIDHAQREPLSSETQYSVDTRRPAPQKALYSTAMESIQGEARRGGTMETDDHMGGIPARNSKGERLLLYIGIIDILQSYRFVKKLEHSWKALVHDGDTVSVHRPGFYAERFQRFMCNTVFKKIPCVHLGRPDVLPQTPPLEEISEGSPIPDPSFSPLVGETLQMLTTSTTLEKLEVAESEFTH
-
分子量
82.5 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PIP5K1A (Phosphatidylinositol-5-phosphate 4-kinase type 1 alpha) is a crucial enzyme involved in phosphoinositide metabolism, playing a significant role in cell signaling processes. It catalyzes the conversion of phosphatidylinositol-5-phosphate (PI5P) to phosphatidylinositol 4,5-bisphosphate (PIP2), a key component that regulates various cellular functions, including membrane trafficking, cytoskeletal dynamics, and signal transduction pathways. Dysregulation of PIP5K1A has been implicated in several pathological conditions, including cancer progression and neurodegenerative diseases, making it a target of interest for therapeutic development. Researchers aim to elucidate the functional mechanisms of PIP5K1A through recombinant protein studies, which enable the analysis of its enzymatic activity, interaction with other proteins, and contribution to cellular processes. By producing and characterizing recombinant PIP5K1A, scientists can investigate its structural properties, regulatory patterns, and biological roles in greater detail, potentially leading to novel insights into its function in health and disease. Understanding the dynamics of PIP5K1A at a molecular level is vital for developing strategies to manipulate its activity for therapeutic interventions, thereby offering promising avenues for the treatment of diseases linked to phosphoinositide signaling dysregulation.
