Analytical Data
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Gene name
PIGO
- Application
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Alternative Names
PIGO; UNQ632/PRO1249; GPI ethanolamine phosphate transferase 3; EC 2.-.-.-; Phosphatidylinositol-glycan biosynthesis class O protein; PIG-O
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8TEQ8
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Expression Region
1-454 aa
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AA Sequence
MDSGEWEVSNQHVLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLVERLENDTLLVVAGDHGMTTNGDHGGDSELEVSAALFLYSPTAVFPSTPPEEPEVIPQVSLVPTLALLLGLPIPFGNIGEVMAELFSGGEDSQPHSSALAQASALHLNAQQVSRFLHTYSAATQDLQAKELHQLQNLFSKASADYQWLLQSPKGAEATLPTVIAELQQFLRGARAMCIESWARFSLSFLLLHLLAAGIPVTTPGPFTVPWQAVSAWALMATQTFYSTGHQPVFPAIHWHAAFVGFPEGHGSCTWLPALLVGANTFASHLLFAVGCPLLLLWPFLCESQGLRKRQQPPGNEADARVRPEEEEEPLMEMRLRDAPQHFYAALLQLGLKYLFILGIQILACALAASILRRHLMVWKVFAPKFIFEAVGFIVSSVGLLLGIALVMRVDGAVSSWFRQLFLAQQR
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Molecular Weight
75.68 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PIGO (Phosphatidylinositol Glycan Class O) is a crucial protein involved in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors, which are essential for the membrane attachment of various proteins in eukaryotic cells. Research into PIGO is motivated by its significant role in cell signaling, immune responses, and the pathology of several diseases, including cancer and neurodegenerative disorders. PIGO participates in anchoring proteins that modulate cellular processes, making it a target of interest in understanding both normal physiological functions and disease mechanisms. Dysregulation of PIGO and related pathways can lead to altered protein localization and function, contributing to abnormal cell behavior. Moreover, the study of PIGO and its interactions may pave the way for therapeutic interventions by enhancing our knowledge of GPI anchor biosynthesis and its implications in health and disease. Given the complexity of GPI-anchored proteins and their diverse functionalities, ongoing studies aim to elucidate the structural and functional characteristics of PIGO, providing insights into its regulatory mechanisms and potential roles in therapeutic applications.
