Analytical Data
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Gene name
PI4K2A
- Application
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Alternative Names
PI4K2A; Phosphatidylinositol 4-kinase type 2-alpha; EC 2.7.1.67; Phosphatidylinositol 4-kinase type II-alpha
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9BTU6
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Expression Region
1-479 aa
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AA Sequence
MDETSPLVSP ERAQPPDYTF PSGSGAHFPQ VPGGAVRVAA AAGSGPSPPG SPGHDRERQP LLDRARGAAA QGQTQTVAAQ AQALAAQAAA AAHAAQAHRE RNEFPEDPEF EAVVRQAELA IERCIFPERI YQGSSGSYFV KDPQGRIIAV FKPKNEEPYG HLNPKWTKWL QKLCCPCCFG RDCLVLNQGY LSEAGASLVD QKLELNIVPR TKVVYLASET FNYSAIDRVK SRGKRLALEK VPKVGQRFNR IGLPPKVGSF QLFVEGYKDA DYWLRRFEAE PLPENTNRQL LLQFERLVVL DYIIRNTDRG NDNWLIKYDC PMDSSSSRDT DWVVVKEPVI KVAAIDNGLA FPLKHPDSWR AYPFYWAWLP QAKVPFSQEI KDLILPKISD PNFVKDLEED LYELFKKDPG FDRGQFHKQI AVMRGQILNL TQALKDNKSP LHLVQMPPVI VETARSHQRS SSESYTQSFQ SRKPFFSWW
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Molecular Weight
54.0 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PI4K2A (Phosphatidylinositol 4-kinase type 2 alpha) is a critical enzyme involved in lipid metabolism, particularly in the synthesis of phosphatidylinositol 4-phosphate (PI4P), a pivotal phospholipid in cellular signaling and membrane dynamics. Research into PI4K2A is gaining momentum due to its involvement in various cellular processes, including vesicle trafficking, endocytosis, and the regulation of signaling pathways. Dysregulation of PI4K2A has been implicated in several diseases, including cancer and neurodegenerative disorders, highlighting its potential as a therapeutic target. The reconstitution of PI4K2A as a recombinant protein allows for detailed studies of its biochemical properties, enzymatic activity, and interaction with other molecules. This is vital for understanding its role in disease pathology and the development of inhibitors that can modulate its function. The research into recombinant PI4K2A not only provides insights into its mechanisms of action but also facilitates the exploration of its functions within complex biological systems, thus paving the way for novel therapeutic strategies. The ability to produce PI4K2A in a controlled environment enhances the feasibility of conducting high-throughput screening of small molecules that could serve as potential drugs, making it a focal point of interest in both basic and applied biomedical research.
