Analytical Data
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Gene name
PHF7
- Application
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Alternative Names
DKFZp434L1850; HSPC045; HSPC226 ; MGC26088; NYD SP6; OTTHUMP00000212988; OTTHUMP00000212989; OTTHUMP00000212990; OTTHUMP00000212993; PHD finger protein 7; Phf7; PHF7_HUMAN; Testis development protein NYD SP6 ; Testis development protein NYD-SP6
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9BWX1
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Expression Region
1-381 aa
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AA Sequence
MKTVKEKKEC QRLRKSAKTR RVTQRKPSSG PVCWLCLREP GDPEKLGEFL QKDNISVHYF CLILSSKLPQ RGQSNRGFHG FLPEDIKKEA ARASRKICFV CKKKGAAINC QKDQCLRNFH LPCGQERGCL SQFFGEYKSF CDKHRPTQNI QHGHVGEESC ILCCEDLSQQ SVENIQSPCC SQAIYHRKCI QKYAHTSAKH FFKCPQCNNR KEFPQEMLRM GIHIPDRDAA WELEPGAFSD LYQRYQHCDA PICLYEQGRD SFEDEGRWCL ILCATCGSHG THRDCSSLRS NSKKWECEEC SPAAATDYIP ENSGDIPCCS STFHPEEHFC RDNTLEENPG LSWTDWPEPS LLEKPESSRG RRSYSWRSKG VRITNSCKKS K
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Molecular Weight
43.7 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PHF7 (PHD finger protein 7) is a member of the PHD finger protein family, which plays crucial roles in epigenetic regulation and gene expression. It contains a PHD (plant homeodomain) finger motif, known to interact with histones and other proteins involved in transcriptional regulation. Recent studies have highlighted PHF7's involvement in various biological processes, including stem cell differentiation, development, and cancer progression. Given its significance in these areas, the functional analysis of PHF7 and the development of recombinant PHF7 protein is essential for elucidating its biological roles. Researchers have focused on producing this protein in heterologous systems, such as E. coli and yeast, to facilitate structure-function studies and assess its interactions with other cellular molecules. Understanding PHF7's binding capabilities and its impact on chromatin dynamics may provide insights into disease mechanisms, particularly in cancer where epigenetic alterations are prevalent. The study of PHF7 not only furthers our knowledge of the intricate regulatory networks governing gene expression but also holds potential for therapeutic interventions targeting epigenetic modifications in various diseases.
