Analytical Data
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基因名
PFTK1
- Application
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别名
cdk14; CDK14_HUMAN; Cell division protein kinase 14; Cyclin-dependent kinase 14; hPFTAIRE1; KIAA0834; mKIAA0834; PFTAIRE protein kinase 1; PFTAIRE1; PFTK1; Serine/threonine protein kinase PFTAIRE 1; Serine/threonine-protein kinase PFTAIRE-1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O94921
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表达区间
1-469 aa
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氨基酸序列
MCDLIEPQPA EKIGKMKKLR RTLSESFSRI ALKKDDTTFD EICVTKMSTR NCQGMDSVIK PLDTIPEDKK VRVQRTQSTF DPFEKPANQV KRVHSENNAC INFKTSSTGK ESPKVRRHSS PSSPTSPKFG KADSYEKLEK LGEGSYATVY KGKSKVNGKL VALKVIRLQE EEGTPFTAIR EASLLKGLKH ANIVLLHDII HTKETLTLVF EYVHTDLCQY MDKHPGGLHP DNVKLFLFQL LRGLSYIHQR YILHRDLKPQ NLLISDTGEL KLADFGLARA KSVPSHTYSN EVVTLWYRPP DVLLGSTEYS TCLDMWGVGC IFVEMIQGVA AFPGMKDIQD QLERIFLVLG TPNEDTWPGV HSLPHFKPER FTLYSSKNLR QAWNKLSYVN HAEDLASKLL QCSPKNRLSA QAALSHEYFS DLPPRLWELT DMSSIFTVPN VRLQPEAGES MRAFGKNNSY GKSLSNSKH
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分子量
53.0 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PFTK1, or Pseudomonas fluorescens Thymidine Kinase 1, has emerged as a significant focus in molecular biology and biochemistry research due to its essential role in cell proliferation and the regulation of the cell cycle. As a member of the thymidine kinase family, PFTK1 catalyzes the phosphorylation of thymidine to generate thymidine monophosphate, a critical step in DNA synthesis and repair mechanisms. The enzyme's dysregulation has been implicated in various diseases, including cancer, where abnormal cell division occurs. Consequently, understanding the functional mechanisms and biochemical properties of PFTK1 can provide valuable insights into cell growth regulation and therapeutic targets for cancer treatment. Recent studies have sought to produce recombinant PFTK1 protein to facilitate detailed structural-functional analyses, enabling researchers to explore its enzymatic activity, interaction with substrates, and response to potential inhibitors. Additionally, the recombinant protein can serve as a tool for high-throughput screening of small molecules that could modulate its activity, offering a pathway for innovative cancer therapies. This research is particularly relevant given the pressing need for novel therapeutic options in oncology, where traditional treatments often face limitations due to resistance and side effects. Thus, PFTK1 recombinant protein studies not only contribute to our fundamental understanding of cellular processes but also hold promise for the development of targeted and effective treatments in cancer care.
