Analytical Data
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Gene name
ATG4D
- Application
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Alternative Names
ATG4D_HUMAN; Atg4dl; AUT like 4 cysteine endopeptidase; AUT-like 4 cysteine endopeptidase
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q86TL0
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Expression Region
1-141aa
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AA Sequence
MGGKPRHSLYFIGYQDDFLLYLDPHYCQPTVDVSQADFPLESFHCTSPRKMAFAKMDPSCTVGFYAGDRKEFETLCSELTRVLSSSSATERYPMFTLAEGHAQDHSLDDLCSQLAQPTLRLPRTGRLLRAKRPSSEDFVFL
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Molecular Weight
42.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ATG4D is a member of the autophagy-related protein family, playing a crucial role in the regulation of the autophagy process, which is vital for cellular homeostasis and response to stress. Recent studies have highlighted its involvement in the enzymatic cleavage of the LC3 (microtubule-associated protein 1 light chain 3) precursor, facilitating its conversion into the lipidated form, LC3-II, which is essential for autophagosome formation and maturation. Dysregulation of autophagy and related proteins has been implicated in various diseases, including neurodegenerative disorders, cancer, and infections. The study of ATG4D recombinant protein aims to elucidate its structure-function relationship and its precise role in the autophagic pathway. Additionally, understanding how ATG4D interacts with other autophagy-related proteins can provide insights into the mechanistic pathways governing autophagy and its regulatory networks. By examining the biochemical properties and functional implications of ATG4D, researchers hope to uncover potential therapeutic targets for manipulating autophagy in disease contexts, ultimately contributing to the development of novel strategies for treating conditions associated with impaired autophagic processes. As such, ATG4D represents a significant focal point in the ongoing research into autophagy and its implications for human health and disease.
