Analytical Data
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Gene name
SA
- Application
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Alternative Names
SA；SA2；Cohesin subunit SA-2
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P33402
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Expression Region
1-732aa
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AA Sequence
MSRRKISSES FSSLGSDYLE TSPEEEGECP LSRLCWNGSR SPPGPLEPSP AAAAAAAAPA PTPAASAAAA AATAGARRVQ RRRRVNLDSL GESISRLTAP SPQTIQQTLK RTLQYYEHQV IGYRDAEKNF HNISNRCSYA DHSNKEEIED VSGILQCTAN ILGLKFEEIQ KRFGEEFFNI CFHENERVLR AVGGTLQDFF NGFDALLEHI RTSFGKQATL ESPSFLCKEL PEGTLMLHYF HPHHIVGFAM LGMIKAAGKK IYRLDVEVEQ VANEKLCSDV SNPGNCSCLT FLIKECENTN IMKNLPQGTS QVPADLRISI NTFCRAFPFH LMFDPSMSVL QLGEGLRKQL RCDTHKVLKF EDCFEIVSPK VNATFERVLL RLSTPFVIRT KPEASGSENK DKVMEVKGQM IHVPESNSIL FLGSPCVDKL DELMGRGLHL SDIPIHDATR DVILVGEQAK AQDGLKKRMD KLKATLERTH QALEEEKKKT VDLLYSIFPG DVAQQLWQGQ QVQARKFDDV TMLFSDIVGF TAICAQCTPM QVISMLNELY TRFDHQCGFL DIYKVETIGD AYCVAAGLHR KSLCHAKPIA LMALKMMELS EEVLTPDGRP IQMRIGIHSG SVLAGVVGVR MPRYCLFGNN VTLASKFESG SHPRRINVSP TTYQLLKREE SFTFIPRSRE ELPDNFPKEI PGICYFLEVR TGPKPPKPSL SSSRIKKVSY NIGTMFLRET SL
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Molecular Weight
81.7 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
SA (Streptavidin) recombinant proteins have gained significant attention in molecular biology and biochemistry due to their strong and specific binding affinity for biotin, a key vitamin involved in various enzymatic reactions. The study of SA recombinant proteins emerged from the need for reliable tools in protein purification, detection, and labeling techniques, particularly in the fields of biotechnology and diagnostics. Streptavidin, originally derived from the bacterium *Streptomyces avidinii*, forms a stable complex with biotin that is characterized by an exceptionally high affinity (Kd in the order of 10^-14 M), making it a valuable asset for applications such as ELISA, Western blotting, and the development of biosensors. Advances in recombinant DNA technology have allowed researchers to produce large quantities of recombinant streptavidin with tailored properties, enhancing its utility in various experimental settings. Furthermore, genetic engineering techniques enable the modification of SA to improve its properties, such as solubility, stability, and binding capabilities. These recombinant proteins are also employed in targeted drug delivery systems and imaging techniques, facilitating the visualization of cellular processes. Consequently, the continued study and optimization of SA recombinant proteins hold promise for advancing research methodologies and therapeutic applications, underscoring their importance in both basic and applied sciences.
