Analytical Data
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基因名
UgDH
- Application
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别名
UgDH；UDP-glucose 6-dehydrogenase
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O60701
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表达区间
1-494aa
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氨基酸序列
MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSELEMFEIKKICCIG AGYVGGPTCSVIAHMCPEIRVTVVDVNESRINAWNSPTLPIYEPGLKEVV ESCRGKNLFFSTNIDDAIKEADLVFISVNTPTKTYGMGKGRAADLKYIEA CARRIVQNSNGYKIVTEKSTVPVRAAESIRRIFDANTKPNLNLQVLSNPE FLAEGTAIKDLKNPDRVLIGGDETPEGQRAVQALCAVYEHWVPREKILTT NTWSSELSKLAANAFLAQRISSINSISALCEATGADVEEVATAIGMDQRI GNKFLKASVGFGGSCFQKDVLNLVYLCEALNLPEVARYWQQVIDMNDYQR RRFASRIIDSLFNTVTDKKIAILGFAFKKDTGDTRESSSIYISKYLMDEG AHLHIYDPKVPREQIVVDLSHPGVSEDDQVSRLVTISKDPYEACDGAHAV VICTEWDMFKELDYERIHKKMLKPAFIFDGRRVLDGLHNELQTIGFQIET IGKKVSSKRIPYAPSGEIPKFSLQDPPNKKPKV
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分子量
60 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
UgDH, or Uroporphyrinogen Decarboxylase, is a critical enzyme in the heme biosynthesis pathway, catalyzing the decarboxylation of uroporphyrinogen to coproporphyrinogen. This enzyme plays a crucial role in the production of heme, an essential component for various biological processes, including oxygen transport and cellular respiration. Deficiencies or mutations in UgDH can lead to congenital erythropoietic porphyria, a rare genetic disorder characterized by an accumulation of porphyrins and associated with serious health issues such as photosensitivity and hemolytic anemia. Research on recombinant UgDH has gained momentum owing to its potential applications in therapeutic strategies and industrial biotechnology. By producing and characterizing the recombinant form of UgDH, scientists aim to better understand its structure-function relationships, regulatory mechanisms, and interaction with substrates. Furthermore, recombinant UgDH can be utilized for enzyme replacement therapies in affected individuals and in the development of biosensors for porphyrin detection. The exploration of UgDH not only contributes to our understanding of porphyrin metabolism but also opens avenues for innovative approaches in treating related disorders and harnessing the enzyme's properties for biotechnological advancements. Overall, the study of UgDH is essential for unraveling the complexities of heme biosynthesis and its implications in human health and disease.
