Analytical Data
-
基因名
PDE4A
- Application
-
别名
3'.5'-cyclic-AMP phosphodiesterase 4A. EC:3.1.4.53. DPDE2. PDE46. cAMP-specific phosphodiesterase 4A
-
种属
Human
-
表达系统
E. coli
-
标签
GST-tag at N-terminal
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
P27815
-
表达区间
1-647 aa
-
氨基酸序列
MPLVDFFCETCSKPWLVGWWDQFKRMLNRELTHLSEMSRSGNQVSEYISTTFLDKQNEVEIPSPTMKEREKQQAPRPRPSQPPPPPVPHLQPMSQITGLKKLMHSNSLNNSNIPRFGVKTDQEELLAQELENLNKWGLNIFCVSDYAGGRSLTCIMYMIFQERDLLKKFRIPVDTMVTYMLTLEDHYHADVAYHNSLHAADVLQSTHVLLATPALDAVFTDLEILAALFAAAIHDVDHPGVSNQFLINTNSELALMYNDESVLENHHLAVGFKLLQEDNCDIFQNLSKRQRQSLRKMVIDMVLATDMSKHMTLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLRNMVHCADLSNPTKPLELYRQWTDRIMAEFFQQGDRERERGMEISPMCDKHTASVEKSQVGFIDYIVHPLWETWADLVHPDAQEILDTLEDNRDWYYSAIRQSPSPPPEEESRGPGHPPLPDKFQFELTLEEEEEEEISMAQIPCTAQEALTAQGLSGVEEALDATIAWEASPAQESLEVMAQEASLEAELEAVYLTQQAQSTGSAPVAPDEFSSREEFVVAVSHSSPSALALQSPLLPAWRTLSVSEHAPGLPGLPSTAAEVEAQREHQAAKRACSACAGTFGEDTSALPAPGGGGSGGDPT
-
分子量
98.6 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PDE4A, or phosphodiesterase 4A, is an important enzyme that plays a crucial role in the degradation of cyclic adenosine monophosphate (cAMP), a key signaling molecule involved in various biological processes. The dysfunction of PDE4A has been linked to several diseases, including inflammation, neurological conditions, and respiratory disorders, making it a significant target for therapeutic intervention. Research on PDE4A recombinant proteins has gained momentum due to their potential applications in drug development and understanding cellular signaling pathways. By producing and characterizing these recombinant proteins, scientists aim to elucidate the enzyme's function, regulation, and interactions with other signaling molecules. Furthermore, PDE4A inhibitors have emerged as promising candidates in treating conditions like asthma and chronic obstructive pulmonary disease (COPD) by enhancing cAMP levels and promoting anti-inflammatory effects. The ongoing studies focus on optimizing the expression systems for PDE4A, understanding its structural properties, and investigating its role in pathophysiological processes. Overall, the study of PDE4A recombinant proteins is pivotal for advancing our knowledge of cAMP signaling and developing novel therapeutic strategies.
