Analytical Data
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Gene name
G6PD
- Application
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Alternative Names
G6PD；Glucose-6-phosphate 1-dehydrogenase
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P11413
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Expression Region
2-515aa
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AA Sequence
AEQVALSRTQVCGILREELFQGDAFHQSDTHIFIIMGASGDLAKKKIYPTIWWLFRDGLLPENTFIVGYARSRLTVADIRKQSEPFFKATPEEKLKLEDFFARNSYVAGQYDDAASYQRLNSHMNALHLGSQANRLFYLALPPTVYEAVTKNIHESCMSQIGWNRIIVEKPFGRDLQSSDRLSNHISSLFREDQIYRIDHYLGKEMVQNLMVLRFANRIFGPIWNRDNIACVILTFKEPFGTEGRGGYFDEFGIIRDVMQNHLLQMLCLVAMEKPASTNSDDVRDEKVKVLKCISEVQANNVVLGQYVGNPDGEGEATKGYLDDPTVPRGSTTATFAAVVLYVENERWDGVPFILRCGKALNERKAEVRLQFHDVAGDIFHQQCKRNELVIRVQPNEAVYTKMMTKKPGMFFNPEESELDLTYGNRYKNVKLPDAYERLILDVFCGSQMHFVRSDELREAWRIFTPLLHQIELEKPKPIPYIYGSRGPTEADELMKRVGFQYEGTYKWVNPHKL
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Molecular Weight
60.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
G6PD (Glucose-6-Phosphate Dehydrogenase) is a crucial enzyme in the pentose phosphate pathway, playing a significant role in cellular metabolism by maintaining the NADPH levels necessary for various biosynthetic processes and protecting against oxidative stress. Deficiency in G6PD leads to hemolytic anemia, a condition that affects millions worldwide, especially in regions with high malaria prevalence, where the deficiency provides a selective advantage. Research into recombinant G6PD protein has gained momentum due to its potential applications in diagnostics, therapeutics, and understanding the enzyme's structural and functional properties. By utilizing recombinant DNA technology, scientists can produce large quantities of the G6PD enzyme, facilitating detailed studies on its catalytic mechanisms and the effects of different mutations linked to G6PD deficiency. Additionally, insights gained from these studies could drive the development of enzyme replacement therapies or novel pharmacological approaches to manage G6PD deficiency. The exploration of recombinant G6PD also extends to its role in metabolic pathways and its interaction with various substrates and inhibitors, enriching our understanding of metabolic diseases and the enzyme's evolutionary significance. Consequently, the research on recombinant G6PD protein not only enhances our understanding of this critical enzyme but also opens new avenues for clinical intervention strategies targeting G6PD-related disorders.
