Analytical Data
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Gene name
RARS2
- Application
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Alternative Names
RARS2；RARSL；Probable arginine--tRNA ligase. mitochondrial
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q5T160
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Expression Region
17-578aa
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AA Sequence
LNLP PENLITSISA VPISQKEEVA DFQLSVDSLL EKDNDHSRPD IQVQAKRLAE KLRCDTVVSE ISTGQRTVNF KINRELLTKT VLQQVIEDGS KYGLKSELFS GLPQKKIVVE FSSPNVAKKF HVGHLRSTII GNFIANLKEA LGHQVIRINY LGDWGMQFGL LGTGFQLFGY EEKLQSNPLQ HLFEVYVQVN KEAADDKSVA KAAQEFFQRL ELGDVQALSL WQKFRDLSIE EYIRVYKRLG VYFDEYSGES FYREKSQEVL KLLESKGLLL KTIKGTAVVD LSGNGDPSSI CTVMRSDGTS LYATRDLAAA IDRMDKYNFD TMIYVTDKGQ KKHFQQVFQM LKIMGYDWAE RCQHVPFGVV QGMKTRRGDV TFLEDVLNEI QLRMLQNMAS IKTTKELKNP QETAERVGLA ALIIQDFKGL LLSDYKFSWD RVFQSRGDTG VFLQYTHARL HSLEETFGCG YLNDFNTACL QEPQSVSILQ HLLRFDEVLY KSSQDFQPRH IVSYLLTLSH LAAVAHKTLQ IKDSPPEVAG ARLHLFKAVR SVLANGMKLL GITPVCRM
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Molecular Weight
65 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
RARS2 (Arginyl-tRNA synthetase 2) is an essential mitochondrial enzyme that plays a crucial role in mitochondrial protein synthesis by charging tRNA with the amino acid arginine. Mutations in the RARS2 gene have been linked to various mitochondrial disorders, particularly affecting neurodevelopment and causing significant clinical manifestations such as progressive neurodegeneration, muscle weakness, and sensorineural hearing loss. Research on RARS2 recombinant protein is of increasing interest, as understanding its structure and function can provide insights into the underlying mechanisms of mitochondrial diseases. The study of RARS2 is pivotal in elucidating how defects in mitochondrial protein synthesis contribute to pathology, potentially opening avenues for targeted therapies. Recent advancements in molecular biology techniques allow for the efficient production and purification of RARS2 recombinant proteins, facilitating detailed biochemical and structural studies. By investigating the enzymatic activity, binding properties, and interaction networks of RARS2, researchers aim to gain a deeper understanding of its role in mitochondrial function and the impact of disease-associated mutations. This research ultimately seeks to contribute to the development of therapeutic strategies aimed at alleviating the symptoms of disorders associated with mitochondrial dysfunction, emphasizing the importance of RARS2 in both fundamental and clinical research within mitochondrial biology.
