Analytical Data
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基因名
RARS2
- Application
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别名
RARS2；RARSL；Probable arginine--tRNA ligase. mitochondrial
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q5T160
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表达区间
17-578aa
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氨基酸序列
LNLP PENLITSISA VPISQKEEVA DFQLSVDSLL EKDNDHSRPD IQVQAKRLAE KLRCDTVVSE ISTGQRTVNF KINRELLTKT VLQQVIEDGS KYGLKSELFS GLPQKKIVVE FSSPNVAKKF HVGHLRSTII GNFIANLKEA LGHQVIRINY LGDWGMQFGL LGTGFQLFGY EEKLQSNPLQ HLFEVYVQVN KEAADDKSVA KAAQEFFQRL ELGDVQALSL WQKFRDLSIE EYIRVYKRLG VYFDEYSGES FYREKSQEVL KLLESKGLLL KTIKGTAVVD LSGNGDPSSI CTVMRSDGTS LYATRDLAAA IDRMDKYNFD TMIYVTDKGQ KKHFQQVFQM LKIMGYDWAE RCQHVPFGVV QGMKTRRGDV TFLEDVLNEI QLRMLQNMAS IKTTKELKNP QETAERVGLA ALIIQDFKGL LLSDYKFSWD RVFQSRGDTG VFLQYTHARL HSLEETFGCG YLNDFNTACL QEPQSVSILQ HLLRFDEVLY KSSQDFQPRH IVSYLLTLSH LAAVAHKTLQ IKDSPPEVAG ARLHLFKAVR SVLANGMKLL GITPVCRM
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分子量
65 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RARS2 (Arginyl-tRNA synthetase 2) is an essential mitochondrial enzyme that plays a crucial role in mitochondrial protein synthesis by charging tRNA with the amino acid arginine. Mutations in the RARS2 gene have been linked to various mitochondrial disorders, particularly affecting neurodevelopment and causing significant clinical manifestations such as progressive neurodegeneration, muscle weakness, and sensorineural hearing loss. Research on RARS2 recombinant protein is of increasing interest, as understanding its structure and function can provide insights into the underlying mechanisms of mitochondrial diseases. The study of RARS2 is pivotal in elucidating how defects in mitochondrial protein synthesis contribute to pathology, potentially opening avenues for targeted therapies. Recent advancements in molecular biology techniques allow for the efficient production and purification of RARS2 recombinant proteins, facilitating detailed biochemical and structural studies. By investigating the enzymatic activity, binding properties, and interaction networks of RARS2, researchers aim to gain a deeper understanding of its role in mitochondrial function and the impact of disease-associated mutations. This research ultimately seeks to contribute to the development of therapeutic strategies aimed at alleviating the symptoms of disorders associated with mitochondrial dysfunction, emphasizing the importance of RARS2 in both fundamental and clinical research within mitochondrial biology.
