Analytical Data
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Gene name
TYW5
- Application
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Alternative Names
TYW5；C2orf60；tRNA wybutosine-synthesizing Protein 5
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
A2RUC4
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Expression Region
1-315aa
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AA Sequence
MAGQHLPVPR LEGVSREQFM QHLYPQRKPL VLEGIDLGPC TSKWTVDYLS QVGGKKEVKI HVAAVAQMDF ISKNFVYRTL PFDQLVQRAA EEKHKEFFVS EDEKYYLRSL GEDPRKDVAD IRKQFPLLKG DIKFPEFFKE EQFFSSVFRI SSPGLQLWTH YDVMDNLLIQ VTGKKRVVLF SPRDAQYLYL KGTKSEVLNI DNPDLAKYPL FSKARRYECS LEAGDVLFIP ALWFHNVISE EFGVGVNIFW KHLPSECYDK TDTYGNKDPT AASRAAQILD RALKTLAELP EEYRDFYARR MVLHIQDKAY SKNSE
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TYW5 is a critical protein involved in tRNA modification, specifically in the maturation of uridine to pseudouridine in tRNA molecules. This enzymatic activity plays a vital role in proper tRNA functioning and, consequently, in protein synthesis. Recent studies have highlighted the significance of TYW5 in cellular processes and its potential implications in various diseases, including cancer and neurological disorders. The modification of tRNA is essential for maintaining the fidelity of the translation process, and disruptions in TYW5 activity can lead to translational errors and cellular stress. Additionally, researchers have identified that TYW5 interacts with other proteins and cellular pathways, suggesting its broader role in cellular homeostasis and adaptation to environmental changes. Understanding the structure-function relationship of TYW5 and its regulatory mechanisms is crucial for deciphering its biological role and potential as a therapeutic target. As such, ongoing research focuses on elucidating the detailed mechanisms of TYW5, exploring its involvement in specific diseases, and investigating the potential for manipulating TYW5 activity in therapeutic settings. This growing body of knowledge aims to pave the way for innovative approaches in combating diseases linked to tRNA modification dysregulation.
