Analytical Data
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Gene name
ACSS1
- Application
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Alternative Names
ACSS1；SIR2L3；NAD-dependent Protein deacetylase sirtuin-3. mitochondrial
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NUB1
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Expression Region
38-689aa
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AA Sequence
ASGPSGSAPAVAAAAAQPGSYPALSAQAAREPAAFWGPLARDTLVWDTPY HTVWDCDFSTGKIGWFLGGQLNVSVNCLDQHVRKSPESVALIWERDEPGT EVRITYRELLETTCRLANTLKRHGVHRGDRVAIYMPVSPLAVAAMLACAR IGAVHTVIFAGFSAESLAGRINDAKCKVVITFNQGLRGGRVVELKKIVDE AVKHCPTVQHVLVAHRTDNKVHMGDLDVPLEQEMAKEDPVCAPESMGSED MLFMLYTSGSTGMPKGIVHTQAGYLLYAALTHKLVFDHQPGDIFGCVADI GWITGHSYVVYGPLCNGATSVLFESTPVYPNAGRYWETVERLKINQFYGA PTAVRLLLKYGDAWVKKYDRSSLRTLGSVGEPINCEAWEWLHRVVGDSRC TLVDTWWQTETGGICIAPRPSEEGAEILPAMAMRPFFGIVPVLMDEKGSV VEGSNVSGALCISQAWPGMARTIYGDHQRFVDAYFKAYPGYYFTGDGAYR TEGGYYQITGRMDDVINISGHRLGTAEIEDAIADHPAVPESAVIGYPHDI KGEAAFAFIVVKDSAGDSDVVVQELKSMVATKIAKYAVPDEILVVKRLPK TRSGKVMRRLLRKIITSEAQELGDTTTLEDPSIIAEILSVYQKCKDKQAA AK
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Molecular Weight
75 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ACSS1 (Acetyl-CoA synthetase 1) is an enzyme that plays a critical role in cellular metabolism by catalyzing the conversion of acetate into acetyl-CoA, a central metabolic intermediate linked to various biosynthetic pathways and energy production. The research on ACSS1 has gained momentum due to its involvement in several physiological processes and its potential implications in various diseases, including cancer, metabolic disorders, and neurodegenerative conditions. Abnormal regulation of ACSS1 has been associated with altered cellular homeostasis, influencing lipid metabolism, and tumor progression. Recent studies have revealed that ACSS1 is not only pivotal for maintaining cellular energy levels but also plays a significant role in modulating gene expression through acetylation mechanisms. The enzyme's activity can be influenced by metabolic cues, revealing a complex interplay between metabolism and epigenetic regulation. As the understanding of ACSS1's role in health and disease expands, it presents a promising target for therapeutic interventions aimed at modulating metabolic pathways. Consequently, ongoing research efforts are focused on elucidating the molecular mechanisms governing ACSS1 function, its regulation under various metabolic states, and its potential as a biomarker or therapeutic target in metabolic diseases and cancers.
