Cat: PA2000-5567

Recombinant Mouse ARHGAP1 Protein,His

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Analytical Data

  • Gene name

    ARHGAP1

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    Arhgap1; Rho GTPase-activating Protein 1; Rho-type GTPase-activating Protein 1

  • Species

    Mouse

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    Q5FWK3

  • Expression Region

    1-439aa

  • AA Sequence

    MDPLSELQDD LTLDDTSQAL NQLKLASIDE KNWPSDEMPD FPKSDDSKSS SPEPVTHLKW DDPYYDIARH QIVEVAGDDK YGRKIIVFSA CRMPPSHQLD HSKLLGYLKH TLDQYVESDY TLLYLHHGLT SDNKPSLSWL RDAYREFDRK YKKNIKALYI VHPTMFIKTL LILFKPLISF KFGRKIFYVN YLSELSEHVK LEQLGIPRQV LKYDDFLKST QKSPATAPKP MPPRPPLPNQ QFGVSLQHLQ EKSPGQDPIP IVLRETVAYL QAHALTTEGI FRRSANTQVV REVQQKYNMG LPVDFDQYNE LHLPAVILKT FLRELPEPLL TFDLYPHVVG FLNIDESQRV EVTQQVLQTL PEENYQVLHF LTAFLVQISA HCDQNKMTNT NLAVVFGPNL LWAKDAAITL KAINPINTFT KFLLDHQGEL FPSTDAQGV

  • Molecular Weight

    50.4 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

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Protein Description

ARHGAP1 (Rho GTPase-activating protein 1) is a crucial regulator of Rho family GTPases, which are integral to various cellular processes, including cytoskeletal dynamics, cell migration, and adhesion. Dysregulation of Rho GTPases has been implicated in numerous diseases, including cancer, cardiovascular disorders, and neurodegenerative conditions. The study of ARHGAP1 is particularly relevant due to its role in inhibiting the signaling pathways of Rho proteins, thereby influencing cellular functions. Recent research has highlighted the significance of ARHGAP1 in tumor progression and metastasis, making it a potential therapeutic target. Understanding the structure and function of ARHGAP1 through recombinant protein studies can provide insights into its mechanism of action and its interactions with other cellular components. By utilizing techniques such as protein expression, purification, and functional assays, researchers are aiming to elucidate the biochemical properties and regulatory mechanisms of ARHGAP1. Moreover, investigating its role in specific cellular contexts can lead to the development of novel strategies for disease intervention. Overall, the study of ARHGAP1 and its recombinant forms is critical for advancing our knowledge of Rho GTPase regulation and its implications in health and disease.

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