Analytical Data
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Gene name
ARHGAP15
- Application
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Alternative Names
ARHGAP15; BM-024; BM-030; BM-046; Rho GTPase-activating Protein 15; ArhGAP15
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q53QZ3
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Expression Region
1-475aa
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AA Sequence
MQKSTNSDTS VETLNSTRQG TGAVQMRIKN ANSHHDRLSQ SKSMILTDVG KVTEPISRHR RNHSQHILKD VIPPLEQLMV EKEGYLQKAK IADGGKKLRK NWSTSWIVLS SRRIEFYKES KQQALSNMKT GHKPESVDLC GAHIEWAKEK SSRKNVFQIT TVSGNEFLLQ SDIDFIILDW FHAIKNAIDR LPKDSSCPSR NLELFKIQRS SSTELLSHYD SDIKEQKPEH RKSLMFRLHH SASDTSDKNR VKSRLKKFIT RRPSLKTLQE KGLIKDQIFG SHLHKVCERE NSTVPWFVKQ CIEAVEKRGL DVDGIYRVSG NLATIQKLRF IVNQEEKLNL DDSQWEDIHV VTGALKMFFR ELPEPLFPYS FFEQFVEAIK KQDNNTRIEA VKSLVQKLPP PNRDTMKVLF GHLTKIVAKA SKNLMSTQSL GIVFGPTLLR AENETGNMAI HMVYQNQIAE LMLSEYSKIF GSEED
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Molecular Weight
54.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ARHGAP15 (Rho GTPase activating protein 15) is a crucial regulator of the Rho family of GTPases, which are involved in various cellular processes such as cytoskeletal dynamics, cell migration, and adhesion. Research on ARHGAP15 has gained attention due to its potential role in cancer biology, particularly in the context of tumor progression and metastasis. It has been observed that ARHGAP15 can modulate the signaling pathways mediated by Rho proteins, thereby influencing cellular behaviors that are critical for cancerous transformations. The protein is believed to act as a tumor suppressor in certain types of cancer, where its downregulation correlates with increased tumor invasiveness and poor prognosis. Moreover, studies have indicated that ARHGAP15 may be a key player in both the epithelial-to-mesenchymal transition (EMT) and the reorganization of the actin cytoskeleton, processes fundamental to cancer cell dissemination. Understanding the molecular mechanisms by which ARHGAP15 exerts its effects can provide valuable insights into its function and therapeutic potential. Consequently, the development of recombinant ARHGAP15 protein has become an area of interest, enabling researchers to conduct further investigations into its interactions, functional assays, and potential as a target for innovative cancer therapies. This research may not only elucidate the role of ARHGAP15 in cellular signal transduction but also contribute to the identification of new strategies for cancer intervention.
