Cat: PA2000-5558

Recombinant Human ARFGAP2 Protein,His

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Analytical Data

  • Gene name

    ARFGAP2

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    ARFG2_HUMAN; ARFGAP2; FLJ14576; FLJ26000

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    Q8N6H7

  • Expression Region

    2-521aa

  • AA Sequence

    AAEPNKTEI QTLFKRLRAV PTNKACFDCG AKNPSWASIT YGVFLCIDCS GVHRSLGVHL SFIRSTELDS NWNWFQLRCM QVGGNANATA FFRQHGCTAN DANTKYNSRA AQMYREKIRQ LGSAALARHG TDLWIDNMSS AVPNHSPEKK DSDFFTEHTQ PPAWDAPATE PSGTQQPAPS TESSGLAQPE HGPNTDLLGT SPKASLELKS SIIGKKKPAA AKKGLGAKKG LGAQKVSSQS FSEIERQAQV AEKLREQQAA DAKKQAEESM VASMRLAYQE LQIDRKKEEK KLQNLEGKKR EQAERLGMGL VSRSSVSHSV LSEMQVIEQE TPVSAKSSRS QLDLFDDVGT FASGPPKYKD NPFSLGESFG SRWDTDAAWG MDRVEEKEPE VTISSIRPIS ERATNRREVE SRSSGLESSE ARQKFAGAKA ISSDMFFGRE VDAEYEARSR LQQLSGSSAI SSSDLFGDMD GAHGAGSVSL GNVLPTADIA QFKQGVKSVA GKMAVLANGV MNSLQDRYGS Y

  • Molecular Weight

    56.7 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

Quality inspection process

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Protein Description

ARFGAP2 (ADP-ribosylation factor GTPase-activating protein 2) is a key regulator of intracellular membrane traffic and is involved in the recycling and transport of vesicles between various cellular compartments. It plays a critical role in modulating the activity of ADP-ribosylation factors (ARFs), which are essential for the formation, motility, and fusion of transport vesicles. Dysregulation of ARFGAP2 has been implicated in several pathological conditions, including cancer and neurodegenerative diseases, thereby underscoring its significance in cellular homeostasis and disease mechanisms. Recent studies have focused on the structural biology of ARFGAP2 and its interaction with ARF proteins, revealing insights into the molecular mechanisms underlying its function. Understanding the biochemical properties and pathways involving ARFGAP2 can provide valuable perspectives for developing therapeutic strategies targeting related diseases. Given its essential role in cellular trafficking, researchers are increasingly exploring ARFGAP2 as a potential biomarker and therapeutic target, which positions it at the forefront of cell biology and molecular medicine research.

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