Analytical Data
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Gene name
ARFGAP1
- Application
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Alternative Names
ARF GAP 1;ADP-ribosylation factor 1 GTPase-activating Protein;ARF1 GAP;ARF1-directed GTPase-activating Protein
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8N6T3
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Expression Region
1-414aa
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AA Sequence
MASPRTRKVLKEVRVQDENNVCFECGAFNPQWVSVTYGIWICLECSGRHRGLGVHLSFVRSVTMDKWKDIELEKMKAGGNAKFREFLESQEDYDPCWSLQEKYNSRAAALFRDKVVALAEGREWSLESSPAQNWTPPQPRTLPSMVHRVSGQPQSVTASSDKAFEDWLNDDLGSYQGAQGNRYVGFGNTPPPQKKEDDFLNNAMSSLYSGWSSFTTGASRFASAAKEGATKFGSQASQKFWGHKQQPEPASELGHSLNENVLKPAQEKVKEGKIFDDVSSGVSQLASKGVGSKGWRDVTTFFSGKAEGPLDSPSEGHSYQNSGLDHFQNSNIDQSFWETFGSAEPTKTRKSPSSDSWTCADTSTERRSSDSWEVWGSASTNRNSNSDGGEGGEGTKKAVPPAVPTDDGWDNQNW
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Molecular Weight
71.28 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
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Protein Description
ARFGAP1, a member of the ARF GTPase-activating protein family, plays a crucial role in the regulation of membrane trafficking and signaling pathways by catalyzing the hydrolysis of GTP bound to ARF (ADP-ribosylation factor) proteins. These ARF proteins are essential for the formation of vesicles that transport proteins and lipids within cells, thus influencing various cellular processes, including endocytosis, exocytosis, and Golgi function. Dysregulation of ARFGAP1 and the ARF signaling pathway has been implicated in several diseases, including cancer and neurodegenerative disorders. Recent studies have demonstrated that ARFGAP1 can interact with various cellular partners, suggesting its involvement in complex signaling networks beyond membrane trafficking. The recombinant production of ARFGAP1 provides valuable insights into its structure-function relationships, enzymatic activity, and interactions with ARF proteins, which are essential for understanding its biological roles. Furthermore, characterizing ARFGAP1 as a recombinant protein can facilitate the development of therapeutic strategies that target its activity, potentially offering new avenues for treating diseases associated with its dysregulation. As researchers continue to elucidate the various functions of ARFGAP1, the potential for harnessing its role in cellular pathways for therapeutic benefits remains a promising area of investigation.
