Analytical Data
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基因名
ARFGAP1
- Application
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别名
ARF GAP 1;ADP-ribosylation factor 1 GTPase-activating Protein;ARF1 GAP;ARF1-directed GTPase-activating Protein
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q8N6T3
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表达区间
1-414aa
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氨基酸序列
MASPRTRKVLKEVRVQDENNVCFECGAFNPQWVSVTYGIWICLECSGRHRGLGVHLSFVRSVTMDKWKDIELEKMKAGGNAKFREFLESQEDYDPCWSLQEKYNSRAAALFRDKVVALAEGREWSLESSPAQNWTPPQPRTLPSMVHRVSGQPQSVTASSDKAFEDWLNDDLGSYQGAQGNRYVGFGNTPPPQKKEDDFLNNAMSSLYSGWSSFTTGASRFASAAKEGATKFGSQASQKFWGHKQQPEPASELGHSLNENVLKPAQEKVKEGKIFDDVSSGVSQLASKGVGSKGWRDVTTFFSGKAEGPLDSPSEGHSYQNSGLDHFQNSNIDQSFWETFGSAEPTKTRKSPSSDSWTCADTSTERRSSDSWEVWGSASTNRNSNSDGGEGGEGTKKAVPPAVPTDDGWDNQNW
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分子量
71.28 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
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Protein Description
ARFGAP1, a member of the ARF GTPase-activating protein family, plays a crucial role in the regulation of membrane trafficking and signaling pathways by catalyzing the hydrolysis of GTP bound to ARF (ADP-ribosylation factor) proteins. These ARF proteins are essential for the formation of vesicles that transport proteins and lipids within cells, thus influencing various cellular processes, including endocytosis, exocytosis, and Golgi function. Dysregulation of ARFGAP1 and the ARF signaling pathway has been implicated in several diseases, including cancer and neurodegenerative disorders. Recent studies have demonstrated that ARFGAP1 can interact with various cellular partners, suggesting its involvement in complex signaling networks beyond membrane trafficking. The recombinant production of ARFGAP1 provides valuable insights into its structure-function relationships, enzymatic activity, and interactions with ARF proteins, which are essential for understanding its biological roles. Furthermore, characterizing ARFGAP1 as a recombinant protein can facilitate the development of therapeutic strategies that target its activity, potentially offering new avenues for treating diseases associated with its dysregulation. As researchers continue to elucidate the various functions of ARFGAP1, the potential for harnessing its role in cellular pathways for therapeutic benefits remains a promising area of investigation.
