Analytical Data
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基因名
GPR81
- Application
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别名
GPR81；GPR104；GPR81；HCA1；Hydroxycarboxylic acid receptor 1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9BXC0
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表达区间
1-346aa
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氨基酸序列
MYNGSCCRIEGDTISQVMPPLLIVAFVLGALGNGVALCGFCFHMKTWKPSTVYLFNLAVADFLLMICLPFRTDYYLRRRHWAFGDIPCRVGLFTLAMNRAGSIVFLTVVAADRYFKVVHPHHAVNTISTRVAAGIVCTLWALVILGTVYLLLENHLCVQETAVSCESFIMESANGWHDIMFQLEFFMPLGIILFCSFKIVWSLRRRQQLARQARMKKATRFIMVVAIVFITCYLPSVSARLYFLWTVPSSACDPSVHGALHITLSFTYMNSMLDPLVYYFSSPSFPKFYNKLKICSLKPKQPGHSKTQRPEEMPISNLGRRSCISVANSFQSQSDGQWDPHIVEWH
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分子量
39.2 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GPR81, also known as the hydroxycarboxylic acid receptor 1 (HCA1), is a G-protein coupled receptor (GPCR) that plays a critical role in mediating the effects of lactate—a key metabolite produced during anaerobic metabolism. It is predominantly expressed in various tissues, particularly in adipose tissue and the brain, where it is involved in regulating energy metabolism, inflammation, and neuronal signaling. Research indicates that GPR81 is activated by lactate and other hydroxycarboxylic acids, leading to an inhibition of lipolysis in adipocytes and a modulation of insulin sensitivity. Given its pivotal role in metabolic regulation, GPR81 has emerged as a potential therapeutic target for metabolic disorders such as obesity, type 2 diabetes, and cardiovascular diseases. The development of recombinant GPR81 proteins has enabled researchers to elucidate the receptor's structure-function relationships, understand its signaling pathways, and explore drug design opportunities. Additionally, studying the recombinant protein allows for a better understanding of the receptor's role in various physiological processes and aids in the identification of novel pharmacological agents. Overall, the research on GPR81 recombinant proteins holds promise for advancing our knowledge of metabolic regulation and developing targeted therapies for metabolic diseases.
