Analytical Data
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Gene name
APOBEC3D
- Application
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Alternative Names
MNPQIRNPME RMYRDTFYDN FENEPILYGR SYTWLCYEVK IKRGRSNLLW DTGVFRGPVL PKRQSNHRQE VYFRFENHAE MCFLSWFCGN RLPANRRFQI TWFVSWNPCL PCVVKVTKFL AEHPNVTLTI SAARLYYYRD RDWRWVLLRL HKAGARVKIM DYEDFAYCWE NFVCNEGQPF MPWYKFDDNY ASLHRTLKEI LRNPMEAMYP HIFYFHFKNL LKACGRNESW LCFTMEVTKH HSAVFRKRGV FRNQVDPETH CHAERCFLSW FCDDILSPNT NYEVTWYTSW SPCPECAGEV AEFLARHSNV NLTIFTARLC YFWDTDYQEG LCSLSQEGAS VKIMGYKDFV SCWKNFVYSD DEPFKPWKGL QTNFRLLKRR LREILQ
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96AK3
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Expression Region
1-386aa
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AA Sequence
MNPQIRNPME RMYRDTFYDN FENEPILYGR SYTWLCYEVK IKRGRSNLLW DTGVFRGPVL PKRQSNHRQE VYFRFENHAE MCFLSWFCGN RLPANRRFQI TWFVSWNPCL PCVVKVTKFL AEHPNVTLTI SAARLYYYRD RDWRWVLLRL HKAGARVKIM DYEDFAYCWE NFVCNEGQPF MPWYKFDDNY ASLHRTLKEI LRNPMEAMYP HIFYFHFKNL LKACGRNESW LCFTMEVTKH HSAVFRKRGV FRNQVDPETH CHAERCFLSW FCDDILSPNT NYEVTWYTSW SPCPECAGEV AEFLARHSNV NLTIFTARLC YFWDTDYQEG LCSLSQEGAS VKIMGYKDFV SCWKNFVYSD DEPFKPWKGL QTNFRLLKRR LREILQ
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Molecular Weight
46.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
APOBEC3D is a member of the APOBEC family of cytidine deaminases, enzymes that play significant roles in innate immunity and the antiviral response. These proteins are known for their ability to deaminate cytidine residues in viral DNA and RNA, leading to genetic mutations that inhibit viral replication. The specific study of APOBEC3D has gained prominence due to its implications in the defense against retroviruses, particularly HIV. Recent research has demonstrated that APOBEC3D exhibits antiviral activity by introducing mutations in the viral genome upon encapsidation into viral particles, thus hindering viral propagation. Moreover, structural studies reveal that APOBEC3D's functionality is influenced by its conformational dynamics and interactions with other proteins, highlighting the complexity of its role in the immune response. Understanding the mechanisms of APOBEC3D not only elucidates its potential as a therapeutic target for viral infections but also provides insights into broader immune evasion strategies employed by pathogens. Additionally, the evolutionary conservation of APOBEC3D across various species suggests its importance in the host defense framework. As research progresses, the recombinant expression of APOBEC3D allows for detailed biochemical and structural analyses, which are crucial for uncovering its specific mechanisms of action and potential applications in antiviral therapies.
