Analytical Data
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基因名
ORC5L
- Application
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别名
ORC5; ORC5_HUMAN; ORC5L; ORC5P; ORC5T; Origin recognition complex subunit 5; Origin recognition complex subunit 5 like
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O43913
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表达区间
1-435 aa
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氨基酸序列
MPHLENVVLC RESQVSILQS LFGERHHFSF PSIFIYGHTA SGKTYVTQTL LKTLELPHVF VNCVECFTLR LLLEQILNKL NHLSSSEDGC STEITCETFN DFVRLFKQVT TAENLKDQTV YIVLDKAEYL RDMEANLLPG FLRLQELADR NVTVLFLSEI VWEKFRPNTG CFEPFVLYFP DYSIGNLQKI LSHDHPPEYS ADFYAAYINI LLGVFYTVCR DLKELRHLAV LNFPKYCEPV VKGEASERDT RKLWRNIEPH LKKAMQTVYL REISSSQWEK LQKDDTDPGQ LKGLSAHTHV ELPYYSKFIL IAAYLASYNP ARTDKRFFLK HHGKIKKTNF LKKHEKTSNH LLGPKPFPLD RLLAILYSIV DSRVAPTANI FSQITSLVTL QLLTLVGHDD QLDGPKYKCT VSLDFIRAIA RTVNFDIIKY LYDFL
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分子量
50.2 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ORC5L (Origin Recognition Complex Subunit 5 Like) is a crucial component of the eukaryotic origin recognition complex (ORC), which is essential for the initiation of DNA replication. The study of ORC5L has gained significant attention due to its role in the regulation of the cell cycle and its implications in genomic stability. Mutations or dysregulation of ORC components, including ORC5L, have been linked to various diseases, including cancer, highlighting the importance of understanding its molecular function. Furthermore, ORC5L is involved in the recruitment of additional factors necessary for the unwinding of DNA and formation of the pre-replication complex, which is vital for DNA replication to proceed accurately. Recent advancements in recombinant protein techniques have enabled researchers to produce and purify ORC5L for in vitro studies, allowing for the exploration of its biochemical properties, interaction with other replication factors, and its role in chromatin dynamics. By understanding the structure and function of ORC5L, researchers aim to elucidate the molecular mechanisms underlying DNA replication and its regulation, potentially providing insights into novel therapeutic strategies for diseases associated with DNA replication errors. This research is not only fundamental for cell biology but also holds promise for the development of targeted interventions in cancer and other disorders linked to replication stress.
