Analytical Data
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基因名
TIGAR
- Application
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别名
TIGAR；C12orf5；Fructose-2.6-bisphosphatase TIGAR
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9NQ88
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表达区间
1-270aa
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氨基酸序列
MARFALTVVRHGETRFNKEKIIQGQGVDEPLSETGFKQAAAAGIFLNNVKFTHAFSSDLMRTKQTMHGILERSKFCKDMTVKYDSRLRERKYGVVEGKALSELRAMAKAAREECPVFTPPGGETLDQVKMRGIDFFEFLCQLILKEADQKEQFSQGSPSNCLETSLAEIFPLGKNHSSKVNSDSGIPGLAASVLVVSHGAYMRSLFDYFLTDLKCSLPATLSRSELMSVTPNTGMSLFIINFEEGREVKPTVQCICMNLQDHLNGLTETR+GGYGRKKRRQ
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分子量
31.7 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TIGAR (TP53-induced glycolysis and apoptosis regulator) is a vital protein that plays a crucial role in cellular metabolism and responses to stress, particularly in the context of cancer biology. Discovered as a target of the tumor suppressor protein p53, TIGAR is involved in regulating the glycolytic pathway, modulating glucose metabolism, and influencing the apoptotic processes within cells. When activated by p53, TIGAR directs the cellular metabolism toward the pentose phosphate pathway, leading to the production of NADPH, which protects cells from oxidative stress and promotes cell survival under harsh conditions. The dysregulation of TIGAR has been associated with various cancers, where its loss can lead to increased glycolysis and resistance to apoptosis, fostering tumor growth and progression. As a result, TIGAR has garnered attention as a potential biomarker for cancer prognosis and a target for therapeutic interventions. Research efforts are focused on understanding the structural properties of TIGAR and its mechanisms of action, as well as exploring its interactions with other metabolic regulators. The recombination and study of TIGAR protein are critical for elucidating its function in metabolic reprogramming in tumor cells, which may pave the way for novel cancer treatment strategies aimed at restoring normal metabolic control and enhancing the effectiveness of existing therapies.
