Analytical Data
-
Gene name
TIGAR
- Application
-
Alternative Names
TIGAR；C12orf5；Fructose-2.6-bisphosphatase TIGAR
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q9NQ88
-
Expression Region
1-270aa
-
AA Sequence
MARFALTVVRHGETRFNKEKIIQGQGVDEPLSETGFKQAAAAGIFLNNVKFTHAFSSDLMRTKQTMHGILERSKFCKDMTVKYDSRLRERKYGVVEGKALSELRAMAKAAREECPVFTPPGGETLDQVKMRGIDFFEFLCQLILKEADQKEQFSQGSPSNCLETSLAEIFPLGKNHSSKVNSDSGIPGLAASVLVVSHGAYMRSLFDYFLTDLKCSLPATLSRSELMSVTPNTGMSLFIINFEEGREVKPTVQCICMNLQDHLNGLTETR+GGYGRKKRRQ
-
Molecular Weight
31.7 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
TIGAR (TP53-induced glycolysis and apoptosis regulator) is a vital protein that plays a crucial role in cellular metabolism and responses to stress, particularly in the context of cancer biology. Discovered as a target of the tumor suppressor protein p53, TIGAR is involved in regulating the glycolytic pathway, modulating glucose metabolism, and influencing the apoptotic processes within cells. When activated by p53, TIGAR directs the cellular metabolism toward the pentose phosphate pathway, leading to the production of NADPH, which protects cells from oxidative stress and promotes cell survival under harsh conditions. The dysregulation of TIGAR has been associated with various cancers, where its loss can lead to increased glycolysis and resistance to apoptosis, fostering tumor growth and progression. As a result, TIGAR has garnered attention as a potential biomarker for cancer prognosis and a target for therapeutic interventions. Research efforts are focused on understanding the structural properties of TIGAR and its mechanisms of action, as well as exploring its interactions with other metabolic regulators. The recombination and study of TIGAR protein are critical for elucidating its function in metabolic reprogramming in tumor cells, which may pave the way for novel cancer treatment strategies aimed at restoring normal metabolic control and enhancing the effectiveness of existing therapies.
