Analytical Data
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基因名
20E
- Application
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别名
20E；EIF2BB；Translation initiation factor eIF2B subunit beta
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P49770
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表达区间
1-351aa
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氨基酸序列
MPGSAAKGSELSERIESFVETLKRGGGPRSSEEMARETLGLLRQIITDHRWSNAGELMELIRREGRRMTAAQPSETTVGNMVRRVLKIIREEYGRLHGRSDESDQQESLHKLLTSGGLNEDFSFHYAQLQSNIIEAINELLVELEGTMENIAAQALEHIHSNEVIMTIGFSRTVEAFLKEAARKRKFHVIVAECAPFCQGHEMAVNLSKAGIETTVMTDAAIFAVMSRVNKVIIGTKTILANGALRAVTGTHTLALAAKHHSTPLIVCAPMFKLSPQFPNEEDSFHKFVAPEEVLPFTEGDILEKVSVHCPVFDYVPPELITLFISNIGGNAPSYIYRLMSELYHPDDHVL
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分子量
66.0 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The 20E recombinant protein, primarily linked to the juvenile hormone (JH) signaling pathway in insects, has garnered significant interest due to its crucial role in developmental processes and pest management strategies. Ecdysteroids, including 20-hydroxyecdysone (20E), are hormones that regulate various physiological functions such as molting, reproduction, and metabolism in insects. The production of recombinant 20E protein presents a promising avenue for understanding the molecular mechanisms underpinning these processes. Researchers have been exploring the implications of 20E in not only insect physiology but also its potential applications in agriculture; specifically, the ability to manipulate this hormonal pathway could lead to novel pest control methods that minimize chemical pesticide use. Advances in genetic engineering techniques have made it feasible to produce large quantities of bioactive 20E, facilitating in-depth studies on its interactions with nuclear receptors and other target proteins. Overall, ongoing research into 20E recombinant proteins aims to enhance our understanding of insect biology and develop sustainable approaches to pest management, marking a significant contribution to the fields of entomology and agricultural biotechnology.
