Analytical Data
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Gene name
20E
- Application
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Alternative Names
20E；EIF2BB；Translation initiation factor eIF2B subunit beta
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P49770
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Expression Region
1-351aa
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AA Sequence
MPGSAAKGSELSERIESFVETLKRGGGPRSSEEMARETLGLLRQIITDHRWSNAGELMELIRREGRRMTAAQPSETTVGNMVRRVLKIIREEYGRLHGRSDESDQQESLHKLLTSGGLNEDFSFHYAQLQSNIIEAINELLVELEGTMENIAAQALEHIHSNEVIMTIGFSRTVEAFLKEAARKRKFHVIVAECAPFCQGHEMAVNLSKAGIETTVMTDAAIFAVMSRVNKVIIGTKTILANGALRAVTGTHTLALAAKHHSTPLIVCAPMFKLSPQFPNEEDSFHKFVAPEEVLPFTEGDILEKVSVHCPVFDYVPPELITLFISNIGGNAPSYIYRLMSELYHPDDHVL
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Molecular Weight
66.0 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The 20E recombinant protein, primarily linked to the juvenile hormone (JH) signaling pathway in insects, has garnered significant interest due to its crucial role in developmental processes and pest management strategies. Ecdysteroids, including 20-hydroxyecdysone (20E), are hormones that regulate various physiological functions such as molting, reproduction, and metabolism in insects. The production of recombinant 20E protein presents a promising avenue for understanding the molecular mechanisms underpinning these processes. Researchers have been exploring the implications of 20E in not only insect physiology but also its potential applications in agriculture; specifically, the ability to manipulate this hormonal pathway could lead to novel pest control methods that minimize chemical pesticide use. Advances in genetic engineering techniques have made it feasible to produce large quantities of bioactive 20E, facilitating in-depth studies on its interactions with nuclear receptors and other target proteins. Overall, ongoing research into 20E recombinant proteins aims to enhance our understanding of insect biology and develop sustainable approaches to pest management, marking a significant contribution to the fields of entomology and agricultural biotechnology.
