Analytical Data
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基因名
TDP1
- Application
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别名
TDP1；DP1；Transcription factor Dp-1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9NUW8
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表达区间
1-608aa
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氨基酸序列
MSQEGDYGRWTISSSDESEEEKPKPDKPSTSSLLCARQGAANEPRYTCSE AQKAAHKRKISPVKFSNTDSVLPPKRQKSGSQEDLGWCLSSSDDELQPEM PQKQAEKVVIKKEKDISAPNDGTAQRTENHGAPACHRLKEEEDEYETSGE GQDIWDMLDKGNPFQFYLTRVSGVKPKYNSGALHIKDILSPLFGTLVSSA QFNYCFDVDWLVKQYPPEFRKKPILLVHGDKREAKAHLHAQAKPYENISL CQAKLDIAFGTHHTKMMLLLYEEGLRVVIHTSNLIHADWHQKTQGIWLSP LYPRIADGTHKSGESPTHFKADLISYLMAYNAPSLKEWIDVIHKHDLSET NVYLIGSTPGRFQGSQKDNWGHFRLKKLLKDHASSMPNAESWPVVGQFSS VGSLGADESKWLCSEFKESMLTLGKESKTPGKSSVPLYLIYPSVENVRTS LEGYPAGGSLPYSIQTAEKQNWLHSYFHKWSAETSGRSNAMPHIKTYMRP SPDFSKIAWFLVTSANLSKAAWGALEKNGTQLMIRSYELGVLFLPSAFGL DSFKVKQKFFAGSQEPMATFPVPYDLPPELYGSKDRPWIWNIPYVKAPDT HGNMWVPS
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分子量
93 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TDP1 (Tyrosyl-DNA phosphodiesterase 1) is an important enzyme involved in the repair of DNA damage caused by certain types of lesions, particularly those resulting from topoisomerase I activity. Topoisomerase I generates temporary single-strand breaks in DNA to alleviate torsional strain during replication and transcription, but if these breaks are not properly resolved, they can lead to cytotoxic effects and genomic instability. TDP1 plays a crucial role in removing the covalent linkage between topoisomerase I and DNA, thereby facilitating the repair process. Research into TDP1 has gained attention due to its implications in cancer therapy, as inhibiting TDP1 can enhance the effectiveness of topoisomerase inhibitors commonly used in chemotherapy. Additionally, mutations in the TDP1 gene have been associated with a rare neurodegenerative disorder known as SCAN1, which further underscores the enzyme's significance in maintaining genomic integrity. Understanding the structure and function of recombinant TDP1 is essential for developing targeted therapies and improving existing treatment strategies for cancers driven by topoisomerase dysregulation. Experimental studies involving the expression and purification of recombinant TDP1 have provided insights into its enzymatic mechanism and substrate specificity, laying the groundwork for novel anticancer strategies and the potential development of TDP1 inhibitors that can be used to sensitize tumors to conventional therapies. These investigations are vital for advancing our knowledge of DNA repair processes and their roles in disease, particularly in the context of cancer biology and therapeutic resistance.
