Analytical Data
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Gene name
AKAP8
- Application
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Alternative Names
AKAP 8; AKAP 95; AKAP-8; AKAP-95; Akap8; AKAP8_HUMAN; AKAP95
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O43823
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Expression Region
1-692aa
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AA Sequence
MDQGYGGYGA WSAGPANTQG AYGTGVASWQ GYENYNYYGA QNTSVTTGAT YSYGPASWEA AKANDGGLAA GAPAMHMASY GPEPCTDNSD SLIAKINQRL DMMSKEGGRG GSGGGGEGIQ DRESSFRFQP FESYDSRPCL PEHNPYRPSY SYDYEFDLGS DRNGSFGGQY SECRDPARER GSLDGFMRGR GQGRFQDRSN PGTFMRSDPF VPPAASSEPL STPWNELNYV GGRGLGGPSP SRPPPSLFSQ SMAPDYGVMG MQGAGGYDST MPYGCGRSQP RMRDRDRPKR RGFDRFGPDG TGRKRKQFQL YEEPDTKLAR VDSEGDFSEN DDAAGDFRSG DEEFKGEDEL CDSGRQRGEK EDEDEDVKKR REKQRRRDRT RDRAADRIQF ACSVCKFRSF DDEEIQKHLQ SKFHKETLRF ISTKLPDKTV EFLQEYIVNR NKKIEKRRQE LMEKETAKPK PDPFKGIGQE HFFKKIEAAH CLACDMLIPA QPQLLQRHLH SVDHNHNRRL AAEQFKKTSL HVAKSVLNNR HIVKMLEKYL KGEDPFTSET VDPEMEGDDN LGGEDKKETP EEVAADVLAE VITAAVRAVD GEGAPAPESS GEPAEDEGPT DTAEAGSDPQ AEQLLEEQVP CGTAHEKGVP KARSEAAEAG NGAETMAAEA ESAQTRVAPA PAAADAEVEQ TDAESKDAVP TE
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Molecular Weight
76.1 KDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
AKAP8, a member of the A-kinase-anchoring protein family, plays a crucial role in the spatial organization of protein kinases within cells, particularly protein kinase A (PKA). This protein is vital for various cellular processes, including signal transduction, regulating gene expression, and maintaining cellular architecture. Recent studies have indicated that AKAP8 is involved in critical physiological functions, such as cardiac signaling and neuronal communication, due to its ability to compartmentalize signaling molecules effectively. Moreover, dysregulation of AKAP8 has been implicated in several pathological conditions, including cancer and cardiovascular diseases. Understanding AKAP8 at the molecular level, particularly through the study of its recombinant protein, can provide insights into its functional mechanisms and interactions with other signaling proteins. Recombinant AKAP8 can be produced using various expression systems, allowing for detailed studies on its structure-function relationships, interaction networks, and potential as a therapeutic target. The advancement of techniques such as X-ray crystallography and cryo-electron microscopy has enabled researchers to elucidate the 3D structures of AKAP8, revealing key insights into its binding sites and regulatory domains. This knowledge could pave the way for the development of novel strategies aimed at modulating AKAP8 activity in disease contexts. Therefore, the research on AKAP8 recombinant protein not only enhances our understanding of its fundamental biological roles but also holds promise for innovative therapeutic approaches in treating diseases associated with PKA dysregulation.
