Analytical Data
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Gene name
AKAP8L
- Application
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Alternative Names
AKAP8L; NAKAP; NAKAP95; HRIHFB2018A-kinase anchor Protein 8-like
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9ULX6
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Expression Region
1-646aa
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AA Sequence
MSYTGFVQGSETTLQSTYSDTSAQPTCDYGYGTWNSGTNRGYEGYGYGYGYGQDNTTNYGYGMATSHSWEMPSSDTNANTSASGSASADSVLSRINQRLDMVPHLETDMMQGGVYGSGGERYDSYESCDSRAVLSERDLYRSGYDYSELDPEMEMAYEGQYDAYRDQFRMRGNDTFGPRAQGWARDARSGRPMASGYGRMWEDPMGARGQCMSGASRLPSLFSQNIIPEYGMFQGMRGGGAFPGGSRFGFGFGNGMKQMRRTWKTWTTADFRTKKKKRKQGGSPDEPDSKATRTDCSDNSDSDNDEGTEGEATEGLEGTEAVEKGSRVDGEDEEGKEDGREEGKEDPEKGALTTQDENGQTKRKLQAGKKSQDKQKKRQRDRMVERIQFVCSLCKYRTFYEDEMASHLDSKFHKEHFKYVGTKLPKQTADFLQEYVTNKTKKTEELRKTVEDLDGLIQQIYRDQDLTQEIAMEHFVKKVEAAHCAACDLFIPMQFGIIQKHLKTMDHNRNRRLMMEQSKKSSLMVARSILNNKLISKKLERYLKGENPFTDSPEEEKEQEEAEGGALDEGAQGEAAGISEGAEGVPAQPPVPPEPAPGAVSPPPPPPPEEEEEGAVPLLGGALQRQIRGIPGLDVEDDEEGGGGAP
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Molecular Weight
97.46 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
AKAP8L (A-kinase anchoring protein 8-like) is a member of the AKAP family, which plays a critical role in anchoring protein kinase A (PKA) and other signaling molecules to specific cellular compartments, thereby regulating various physiological processes. Research on AKAP8L has gained interest due to its involvement in crucial cellular functions such as cell growth, differentiation, and apoptosis. Abnormal expression or dysfunction of AKAP proteins, including AKAP8L, has been implicated in several diseases, including cancer and cardiovascular disorders. Understanding the structure and function of AKAP8L at the molecular level is essential for elucidating its role in these pathological conditions. One promising approach is the production of recombinant AKAP8L proteins, which can be used in biochemical and cellular assays to dissect its interactions with PKA and other signaling partners. This research can elucidate the regulatory mechanisms underlying AKAP8L-mediated signaling pathways, offering insights into potential therapeutic targets for diseases associated with dysregulated AKAP function. Furthermore, studying the post-translational modifications and structural dynamics of AKAP8L can reveal important information about its role in cellular signaling networks. As such, the recombinant expression of AKAP8L is a critical step in advancing our understanding of its biological functions and therapeutic potential.
